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公开(公告)号：US08778650B2
公开(公告)日：2014-07-15
申请号：US13266525
申请日：2010-04-26
CPC分类号： C11D3/38618 , C11D3/386 , C11D3/38681 , C12N9/54
摘要： The invention is directed to an alkaline protease that has the amino acid sequence of SEQ ID NO: 2 or of an amino acid sequence having an identity of 90% or more thereto, except that one or more amino acid residues at positions selected from (a) position 6, (b) position 15, (c) position 16, (d) position 65, (e) position 66, (f) position 82, (g) position 83, (h) position 204, (i) position 319, and (j) position 337 of SEQ ID NO: 2, or at positions corresponding thereto, are substituted with certain other amino acid residues. The invention is also directed to detergent compositions and culture medium that contain the protease.
摘要翻译： 本发明涉及具有SEQ ID NO：2的氨基酸序列或90％以上的氨基酸序列的碱性蛋白酶，除了选自（a）的位置的一个或多个氨基酸残基 ）位置6，（b）位置15，（c）位置16，（d）位置65，（e）位置66，（f）位置82，（g）位置83，（h）位置204，（i） 319和（j）SEQ ID NO：2的位置337或与其相应的位置被某些其它氨基酸残基取代。 本发明还涉及含有蛋白酶的洗涤剂组合物和培养基。
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公开(公告)号：US20120058928A1
公开(公告)日：2012-03-08
申请号：US13266525
申请日：2010-04-26
CPC分类号： C11D3/38618 , C11D3/386 , C11D3/38681 , C12N9/54
摘要： An alkaline protease variant derived from an alkaline protease consisting of an amino acid sequence represented by SEQ ID NO: 2 or consisting of an amino acid sequence having an identity of 90% or more therewith, which variant has mutations wherein one or more amino acid residues at positions selected from (a) position 6, (b) position 15, (c) position 16, (d) position 65, (e) position 66, (f) position 82, (g) position 83, (h) position 204, (i) position 319, and (j) position 337 of the amino acid sequence represented by SEQ ID NO: 2, or at positions corresponding thereto are substituted with the following amino acid residues: (a) or a position corresponding thereto: Typ, Leu, Val, Ile, Met, Tyr, Gln, Lys, Thr, Phe, Arg, Ser, Cys, Ala, or His; (b) or a position corresponding thereto: Glu, Met, Asp, Val, Gln, Arg, Cys, Trp, Ala, or Phe; (c) or a position corresponding thereto: Met, Glu, Arg, Val, Lys, Phe, Tyr, Ile, His, Asp, or Cys; (d) or a position corresponding thereto: Trp; (e) or a position corresponding thereto: His, Trp, Ser, or Leu; (f) or a position corresponding thereto: Ala, Glu, Gln, Ser, Cys, Gly, H is, Lys, Arg, Met, or Asn; (g) or a position corresponding thereto: Ala, Ser, or Cys; (h) or a position corresponding thereto: Glu, Asp, Cys, Val, Thr, Pro, His, Ile, Trp, Ser, Asn, Lys, or Arg; (i) or a position corresponding thereto: Trp, Val, Thr, Leu, Ile, Cys, Glu, Lys, Tyr, Arg, Phe, Gln, Met, Pro, Asp, Asn, H is, or Ser; and (j) or a position corresponding thereto: Arg, Gly, Ser, Lys, Gln, Thr, His, Ala, Cys, or Val.
摘要翻译： 由碱性蛋白酶衍生的碱性蛋白酶变体，其由SEQ ID NO：2所示的氨基酸序列组成或由与其同一性为90％以上的氨基酸序列组成，所述变体具有突变，其中一个或多个氨基酸残基 在从（a）位置6，（b）位置15，（c）位置16，（d）位置65，（e）位置66，（f）位置82，（g）位置83，（h） 204，（i）位置319和（j）由SEQ ID NO：2表示的氨基酸序列的位置337或与其相对应的位置被以下氨基酸残基取代：（a）或与其对应的位置： Typ，Leu，Val，Ile，Met，Tyr，Gln，Lys，Thr，Phe，Arg，Ser，Cys，Ala或His; （b）或相应位置：Glu，Met，Asp，Val，Gln，Arg，Cys，Trp，Ala或Phe; （c）或对应的位置：Met，Glu，Arg，Val，Lys，Phe，Tyr，Ile，His，Asp或Cys; （d）或与之对应的位置：Trp; （e）或与之对应的位置：His，Trp，Ser或Leu; （f）或对应位置：Ala，Glu，Gln，Ser，Cys，Gly，H is，Lys，Arg，Met或Asn; （g）或对应的位置：Ala，Ser或Cys; （h）或相应位置：Glu，Asp，Cys，Val，Thr，Pro，His，Ile，Trp，Ser，Asn，Lys或Arg; （i）或与其相对应的位置：Trp，Val，Thr，Leu，Ile，Cys，Glu，Lys，Tyr，Arg，Phe，Gln，Met，Pro，Asp，Asn，H is或Ser; 和（j）或对应的位置：Arg，Gly，Ser，Lys，Gln，Thr，His，Ala，Cys或Val。
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公开(公告)号：US07368273B2
公开(公告)日：2008-05-06
申请号：US10385662
申请日：2003-03-12
申请人： Mitsuyoshi Okuda , Tsuyoshi Sato , Kazuhiro Saito , Nobuyuki Sumitomo , Yoshifumi Izawa , Katsuhisa Saeki , Tohru Kobayashi , Masafumi Nomura
发明人： Mitsuyoshi Okuda , Tsuyoshi Sato , Kazuhiro Saito , Nobuyuki Sumitomo , Yoshifumi Izawa , Katsuhisa Saeki , Tohru Kobayashi , Masafumi Nomura
CPC分类号： C11D3/386 , C12N9/54 , C12Y304/21062
摘要： An alkaline protease wherein an amino acid residue at (a) position 65, (b) position 101, (c) position 163, (d) position 17-0, (e) position 171, (f) position 273, (g) position 320, (h) position 359 or (i) position 387 of SEQ. ID NO: 2 or at a position corresponding thereto has been selected from the following amino acid residues: position (a): proline, position (b): asparagine, position (e): histidine, aspartic acid, phenylalanine, lysine, asparagine, seine, isoleucine, leucine, glutamine, threonine and valine, position (d): valine and leucine, position (e): alanine, glutamic acid, glycine and threonine, position (K): isoleucine, glycine and threonine, position (g): phenylalanine, valine, threonine, leucine, isoleucine and glycine, position (h): seine, leucine, valine, isoleucine and glutamine, position (i): alanine, lysine, glutamine, glutamic acid, arginine and histidine. A method to an alkaline protease having activity even in the presence of a highly concentrated fatty acid, and exhibiting excellent detergency for the removal of a complex stain containing protein, sebum and the like, and therefore useful as an ingredient in a detergent.
摘要翻译： （a）位置65，（b）位置101，（c）位置163，（d）位置17-0，（e）位置171，（f）位置273，（g） 位置320，（h）位置359或（i）SEQ ID NO： 位置（a）：脯氨酸，位置（b）：天冬酰胺，位置（e）：组氨酸，天冬氨酸，苯丙氨酸，赖氨酸，天冬酰胺， （e）：丙氨酸，谷氨酸，甘氨酸和苏氨酸，位置（K）：异亮氨酸，甘氨酸和苏氨酸，位置（g）：缬氨酸和亮氨酸， （i）：丙氨酸，赖氨酸，谷氨酰胺，谷氨酸，精氨酸和组氨酸，位置（h）：ine碱，亮氨酸，缬氨酸，异亮氨酸和谷氨酰胺。 一种碱性蛋白酶的方法，即使在高度浓缩的脂肪酸的存在下也具有活性，并且对于去除含有蛋白质，皮脂等的复合污渍具有优异的去污力，因此可用作洗涤剂中的成分。
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公开(公告)号：US06803222B2
公开(公告)日：2004-10-12
申请号：US09985689
申请日：2001-11-05
申请人： Yuji Hatada , Akinori Ogawa , Yasushi Kageyama , Tsuyoshi Sato , Hiroyuki Araki , Nobuyuki Sumitomo , Mitsuyoshi Okuda , Katsuhisa Saeki
发明人： Yuji Hatada , Akinori Ogawa , Yasushi Kageyama , Tsuyoshi Sato , Hiroyuki Araki , Nobuyuki Sumitomo , Mitsuyoshi Okuda , Katsuhisa Saeki
IPC分类号： C12N948
CPC分类号： C12N9/54 , C11D3/38609 , C11D3/38618
摘要： The present invention relates to alkaline proteases having high specific activity and strong oxidant resistance. The present invention also relates to alkaline proteases having excellent detergency that is to be added to a detergent.
摘要翻译： 本发明涉及具有高比活性和强氧化剂抗性的碱性蛋白酶。 本发明还涉及具有优异的去污力的碱性蛋白酶，其被添加到洗涤剂中。
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公开(公告)号：US07371839B2
公开(公告)日：2008-05-13
申请号：US10837566
申请日：2004-05-04
申请人： Yuji Hatada , Akinori Ogawa , Yasushi Kageyama , Tsuyoshi Sato , Hiroyuki Araki , Nobuyuki Sumitomo , Mitsuyoshi Okuda , Katsuhisa Saeki
发明人： Yuji Hatada , Akinori Ogawa , Yasushi Kageyama , Tsuyoshi Sato , Hiroyuki Araki , Nobuyuki Sumitomo , Mitsuyoshi Okuda , Katsuhisa Saeki
CPC分类号： C12N9/54 , C11D3/38609 , C11D3/38618
摘要： The present invention relates to alkaline proteases having high specific activity and strong oxidant resistance. The present invention also relates to alkaline proteases having excellent detergency that are to be added to a detergent.
摘要翻译： 本发明涉及具有高比活性和强氧化剂抗性的碱性蛋白酶。 本发明还涉及具有优异的去污力的碱性蛋白酶，其被添加到洗涤剂中。
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公开(公告)号：US20080177040A1
公开(公告)日：2008-07-24
申请号：US12049022
申请日：2008-03-14
申请人： Mitsuyoshi Okuda , Tsuyoshi Sato , Kazuhiro Saito , Nobuyuki Sumitomo , Yoshifumi Izawa , Katsuhisa Saeki , Tohru Kobayashi , Masafumi Nomura
发明人： Mitsuyoshi Okuda , Tsuyoshi Sato , Kazuhiro Saito , Nobuyuki Sumitomo , Yoshifumi Izawa , Katsuhisa Saeki , Tohru Kobayashi , Masafumi Nomura
IPC分类号： C07K2/00
CPC分类号： C11D3/386 , C12N9/54 , C12Y304/21062
摘要： Provided in the present invention is an alkaline protease wherein an amino acid residue at (a) position 65, (b) position 101, (c) position 163, (d) position 170, (e) position 171, (f) position 273, (g) position 320, (h) position 359 or (i) position 387 of SEQ. ID NO:1 or at a position corresponding thereto has been selected from the following amino acid residues: position (a): proline, position (b): asparagine, position (c): histidine, aspartic acid, phenylalanine, lysine, asparagine, serine, isoleucine, leucine, glutamine, threonine and valine, position (d): valine and leucine, position (e): alanine, glutamic acid, glycine and threonine, position (f): isoleucine, glycine and threonine, position (g): phenylalanine, valine, threonine, leucine, isoleucine and glycine, position (h): serine, leucine, valine, isoleucine and glutamine, position (i): alanine, lysine, glutamine, glutamic acid, arginine and histidine.The present invention makes it possible to efficiently produce and provide alkaline proteases having activity even in the presence of a highly concentrated fatty acid, and exhibiting excellent detergency for the removal of a complex stain containing protein, sebum and the like, and therefore being useful as an enzyme to be incorporated in a detergent.
摘要翻译： 在本发明中提供的是碱性蛋白酶，其中（a）位置65，（b）位置101，（c）位置163，（d）位置170，（e）位置171，（f） ，（g）位置320，（h）位置359或（i）SEQ ID NO： 位置（a）：脯氨酸，位置（b）：天冬酰胺，位置（c）：组氨酸，天冬氨酸，苯丙氨酸，赖氨酸，天冬酰胺， 位置（d）：缬氨酸和亮氨酸，位置（e）：丙氨酸，谷氨酸，甘氨酸和苏氨酸，位置（f）：异亮氨酸，甘氨酸和苏氨酸，位置（g） 位置（h）：丝氨酸，亮氨酸，缬氨酸，异亮氨酸和谷氨酰胺，位置（i）：丙氨酸，赖氨酸，谷氨酰胺，谷氨酸，精氨酸和组氨酸，位置（h）：缬氨酸，缬氨酸，苏氨酸，亮氨酸，异亮氨酸和甘氨酸。 本发明使得可以有效地生产和提供具有活性的碱性蛋白酶，即使在高度浓缩的脂肪酸的存在下也具有优异的去污力来去除含有蛋白质，皮脂等的复合污渍，因此可用作 要掺入洗涤剂中的酶。
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公开(公告)号：US07776578B2
公开(公告)日：2010-08-17
申请号：US12049022
申请日：2008-03-14
申请人： Mitsuyoshi Okuda , Tsuyoshi Sato , Kazuhiro Saito , Nobuyuki Sumitomo , Yoshifumi Izawa , Katsuhisa Saeki , Tohru Kobayashi , Masafumi Nomura
发明人： Mitsuyoshi Okuda , Tsuyoshi Sato , Kazuhiro Saito , Nobuyuki Sumitomo , Yoshifumi Izawa , Katsuhisa Saeki , Tohru Kobayashi , Masafumi Nomura
CPC分类号： C11D3/386 , C12N9/54 , C12Y304/21062
摘要： Provided in the present invention is an alkaline protease wherein an amino acid residue at (a) position 65, (b) position 101, (c) position 163, (d) position 170, (e) position 171, (f) position 273, (g) position 320, (h) position 359 or (i) position 387 of SEQ. ID NO:1 or at a position corresponding thereto has been selected from the following amino acid residues: position (a): proline, position (b): asparagine, position (c): histidine, aspartic acid, phenylalanine, lysine, asparagine, serine, isoleucine, leucine, glutamine, threonine and valine, position (d): valine and leucine, position (e): alanine, glutamic acid, glycine and threonine, position (f): isoleucine, glycine and threonine, position (g): phenylalanine, valine, threonine, leucine, isoleucine and glycine, position (h): serine, leucine, valine, isoleucine and glutamine, position (i): alanine, lysine, glutamine, glutamic acid, arginine and histidine.The present invention makes it possible to efficiently produce and provide alkaline proteases having activity even in the presence of a highly concentrated fatty acid, and exhibiting excellent detergency for the removal of a complex stain containing protein, sebum and the like, and therefore being useful as an enzyme to be incorporated in a detergent.
摘要翻译： 在本发明中提供的是碱性蛋白酶，其中（a）位置65，（b）位置101，（c）位置163，（d）位置170，（e）位置171，（f） ，（g）位置320，（h）位置359或（i）SEQ ID NO： 位置（a）：脯氨酸，位置（b）：天冬酰胺，位置（c）：组氨酸，天冬氨酸，苯丙氨酸，赖氨酸，天冬酰胺， 位置（d）：缬氨酸和亮氨酸，位置（e）：丙氨酸，谷氨酸，甘氨酸和苏氨酸，位置（f）：异亮氨酸，甘氨酸和苏氨酸，位置（g） 位置（h）：丝氨酸，亮氨酸，缬氨酸，异亮氨酸和谷氨酰胺，位置（i）：丙氨酸，赖氨酸，谷氨酰胺，谷氨酸，精氨酸和组氨酸，位置（h）：缬氨酸，缬氨酸，苏氨酸，亮氨酸，异亮氨酸和甘氨酸。 本发明使得可以有效地生产和提供具有活性的碱性蛋白酶，即使在高度浓缩的脂肪酸的存在下也具有优异的去污力来去除含有蛋白质，皮脂等的复合污渍，因此可用作 要掺入洗涤剂中的酶。
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公开(公告)号：US06376227B1
公开(公告)日：2002-04-23
申请号：US09509814
申请日：2000-04-06
申请人： Mikio Takaiwa , Mitsuyoshi Okuda , Katsuhisa Saeki , Hiromi Kubota , Jun Hitomi , Yasushi Kageyama , Shitsuw Shikata , Masafumi Nomura
发明人： Mikio Takaiwa , Mitsuyoshi Okuda , Katsuhisa Saeki , Hiromi Kubota , Jun Hitomi , Yasushi Kageyama , Shitsuw Shikata , Masafumi Nomura
IPC分类号： C12N950
CPC分类号： C12N9/54 , C11D3/386 , C11D3/38609 , C11D3/38618
摘要： An alkaline protease having the following properties; a gene encoding the same; a microorganism producing the same; and washing compositions containing the same; (i) acting over a broad pH value range of 4 to 13 and achieving, at pH 6 to 12, 80% or more the activity at the optimum pH value; (ii) when treated at 40° C. for 30 minutes, being stable over a pH value range of 6 to 11; (iii) having an isoelectric point of about 8.9 to 9.1; and (iv) having casein digesting activity that is not inhibited by oleic acid. The alkaline protease of the present invention is highly stable to various surface active agents and fatty acids, and exhibits high stability to oxidizing agents, and is therefore useful as an enzyme to be used in detergents for automatic dishwashers and laundry detergents, both containing bleaching components.
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公开(公告)号：US07297528B2
公开(公告)日：2007-11-20
申请号：US10784870
申请日：2004-02-24
申请人： Mikio Takaiwa , Mitsuyoshi Okuda , Katsuhisa Saeki , Hiromi Kubota , Jun Hitomi , Yasushi Kageyama , Shitsuw Shikata , Masafumi Nomura
发明人： Mikio Takaiwa , Mitsuyoshi Okuda , Katsuhisa Saeki , Hiromi Kubota , Jun Hitomi , Yasushi Kageyama , Shitsuw Shikata , Masafumi Nomura
CPC分类号： C12N9/54 , C11D3/386 , C11D3/38609 , C11D3/38618
摘要： An alkaline protease having the following properties; a gene encoding the same; a microorganism producing the same; and washing compositions containing the same; (i) acting over a broad pH value range of 4 to 13 and achieving, at pH 6 to 12, 80% or more the activity at the optimum pH value; (ii) when treated at 40° C. for 30 minutes, being stable over a pH value range of 6 to 11; (iii) having an isoelectric point of about 8.9 to 9.1; and (iv) having casein digesting activity that is not inhibited by oleic acid. The alkaline protease of the present invention is highly stable to various surface active agents and fatty acids, and exhibits high stability to oxidizing agents, and is therefore useful as an enzyme to be used in detergents for automatic dishwashers and laundry detergents, both containing bleaching components.
摘要翻译： 具有以下特性的碱性蛋白酶; 编码该基因的基因; 生产该微生物的微生物; 和含有该组合物的洗涤组合物; （i）在4至13的广泛pH值范围内起作用，并且在最佳pH值下达到pH6至12,80％或更高的活性; （ii）当在40℃下处理30分钟时，在6至11的pH值范围内是稳定的; （iii）具有约8.9至9.1的等电点; 和（iv）具有不被油酸抑制的酪蛋白消化活性。 本发明的碱性蛋白酶对各种表面活性剂和脂肪酸高度稳定，对氧化剂表现出高稳定性，因此可用作自动洗碗机和洗衣洗涤剂的洗涤剂中的酶，两者均含有漂白组分 。
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公开(公告)号：US06759228B2
公开(公告)日：2004-07-06
申请号：US09920954
申请日：2001-08-03
申请人： Mikio Takaiwa , Mitsuyoshi Okuda , Katsuhisa Saeki , Hiromi Kubota , Jun Hitomi , Yasushi Kageyama , Shitsuw Shikata , Masafumi Nomura
发明人： Mikio Takaiwa , Mitsuyoshi Okuda , Katsuhisa Saeki , Hiromi Kubota , Jun Hitomi , Yasushi Kageyama , Shitsuw Shikata , Masafumi Nomura
IPC分类号： C12N952
CPC分类号： C12N9/54 , C11D3/386 , C11D3/38609 , C11D3/38618
摘要： An alkaline protease having the following properties; a gene encoding the same; a microorganism producing the same; and washing compositions containing the same; (i) acting over a broad pH value range of 4 to 13 and achieving, at pH 6 to 12, 80% or more the activity at the optimum pH value; (ii) when treated at 40° C. for 30 minutes, being stable over a pH value range of 6 to 11; (iii) having an isoelectric point of about 8.9 to 9.1; and (iv) having casein digesting activity that is not inhibited by oleic acid. The alkaline protease of the present invention is highly stable to various surface active agents and fatty acids, and exhibits high stability to oxidizing agents, and is therefore useful as an enzyme to be used in detergents for automatic dishwashers and laundry detergents, both containing bleaching components.
摘要翻译： 具有以下特性的碱性蛋白酶; 编码该基因的基因; 生产该微生物的微生物; 和含有该组合物的洗涤组合物; （i）在4至13的广泛pH值范围内起作用，并且在最佳pH值下达到pH6至12,80％或更高的活性; （ii）当在40℃下处理30分钟时，在6至11的pH值范围内是稳定的; （iii）具有约8.9至9.1的等电点; 和（iv）具有不被油酸抑制的酪蛋白消化活性。 本发明的碱性蛋白酶对各种表面活性剂和脂肪酸高度稳定，对氧化剂表现出高稳定性，因此可用作自动洗碗机和洗衣洗涤剂的洗涤剂中的酶，两者均含有漂白组分 。
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