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公开(公告)号：US08778650B2
公开(公告)日：2014-07-15
申请号：US13266525
申请日：2010-04-26
CPC分类号： C11D3/38618 , C11D3/386 , C11D3/38681 , C12N9/54
摘要： The invention is directed to an alkaline protease that has the amino acid sequence of SEQ ID NO: 2 or of an amino acid sequence having an identity of 90% or more thereto, except that one or more amino acid residues at positions selected from (a) position 6, (b) position 15, (c) position 16, (d) position 65, (e) position 66, (f) position 82, (g) position 83, (h) position 204, (i) position 319, and (j) position 337 of SEQ ID NO: 2, or at positions corresponding thereto, are substituted with certain other amino acid residues. The invention is also directed to detergent compositions and culture medium that contain the protease.
摘要翻译： 本发明涉及具有SEQ ID NO：2的氨基酸序列或90％以上的氨基酸序列的碱性蛋白酶，除了选自（a）的位置的一个或多个氨基酸残基 ）位置6，（b）位置15，（c）位置16，（d）位置65，（e）位置66，（f）位置82，（g）位置83，（h）位置204，（i） 319和（j）SEQ ID NO：2的位置337或与其相应的位置被某些其它氨基酸残基取代。 本发明还涉及含有蛋白酶的洗涤剂组合物和培养基。
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公开(公告)号：US20120058928A1
公开(公告)日：2012-03-08
申请号：US13266525
申请日：2010-04-26
CPC分类号： C11D3/38618 , C11D3/386 , C11D3/38681 , C12N9/54
摘要： An alkaline protease variant derived from an alkaline protease consisting of an amino acid sequence represented by SEQ ID NO: 2 or consisting of an amino acid sequence having an identity of 90% or more therewith, which variant has mutations wherein one or more amino acid residues at positions selected from (a) position 6, (b) position 15, (c) position 16, (d) position 65, (e) position 66, (f) position 82, (g) position 83, (h) position 204, (i) position 319, and (j) position 337 of the amino acid sequence represented by SEQ ID NO: 2, or at positions corresponding thereto are substituted with the following amino acid residues: (a) or a position corresponding thereto: Typ, Leu, Val, Ile, Met, Tyr, Gln, Lys, Thr, Phe, Arg, Ser, Cys, Ala, or His; (b) or a position corresponding thereto: Glu, Met, Asp, Val, Gln, Arg, Cys, Trp, Ala, or Phe; (c) or a position corresponding thereto: Met, Glu, Arg, Val, Lys, Phe, Tyr, Ile, His, Asp, or Cys; (d) or a position corresponding thereto: Trp; (e) or a position corresponding thereto: His, Trp, Ser, or Leu; (f) or a position corresponding thereto: Ala, Glu, Gln, Ser, Cys, Gly, H is, Lys, Arg, Met, or Asn; (g) or a position corresponding thereto: Ala, Ser, or Cys; (h) or a position corresponding thereto: Glu, Asp, Cys, Val, Thr, Pro, His, Ile, Trp, Ser, Asn, Lys, or Arg; (i) or a position corresponding thereto: Trp, Val, Thr, Leu, Ile, Cys, Glu, Lys, Tyr, Arg, Phe, Gln, Met, Pro, Asp, Asn, H is, or Ser; and (j) or a position corresponding thereto: Arg, Gly, Ser, Lys, Gln, Thr, His, Ala, Cys, or Val.
摘要翻译： 由碱性蛋白酶衍生的碱性蛋白酶变体，其由SEQ ID NO：2所示的氨基酸序列组成或由与其同一性为90％以上的氨基酸序列组成，所述变体具有突变，其中一个或多个氨基酸残基 在从（a）位置6，（b）位置15，（c）位置16，（d）位置65，（e）位置66，（f）位置82，（g）位置83，（h） 204，（i）位置319和（j）由SEQ ID NO：2表示的氨基酸序列的位置337或与其相对应的位置被以下氨基酸残基取代：（a）或与其对应的位置： Typ，Leu，Val，Ile，Met，Tyr，Gln，Lys，Thr，Phe，Arg，Ser，Cys，Ala或His; （b）或相应位置：Glu，Met，Asp，Val，Gln，Arg，Cys，Trp，Ala或Phe; （c）或对应的位置：Met，Glu，Arg，Val，Lys，Phe，Tyr，Ile，His，Asp或Cys; （d）或与之对应的位置：Trp; （e）或与之对应的位置：His，Trp，Ser或Leu; （f）或对应位置：Ala，Glu，Gln，Ser，Cys，Gly，H is，Lys，Arg，Met或Asn; （g）或对应的位置：Ala，Ser或Cys; （h）或相应位置：Glu，Asp，Cys，Val，Thr，Pro，His，Ile，Trp，Ser，Asn，Lys或Arg; （i）或与其相对应的位置：Trp，Val，Thr，Leu，Ile，Cys，Glu，Lys，Tyr，Arg，Phe，Gln，Met，Pro，Asp，Asn，H is或Ser; 和（j）或对应的位置：Arg，Gly，Ser，Lys，Gln，Thr，His，Ala，Cys或Val。
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公开(公告)号：US07981659B2
公开(公告)日：2011-07-19
申请号：US12083539
申请日：2006-09-25
IPC分类号： C12N1/20
CPC分类号： C12N15/75 , C12N9/2417 , C12N9/54 , C12P21/02
摘要： Novel Bacillus subtilis mutant strains having good productivity of various enzymes are provided through extensive analysis of strains that are derived from Bacillus subtilis via gene disruption. The Bacillus subtilis mutant strains according to the present invention have genomic structures prepared by deletion of regions listed in the columns for deficient regions. Each of these Bacillus subtilis mutant strains exerts significantly improved secretory productivity of a protein when a gene encoding such a secretory target protein is introduced so that it can be expressed, compared with a case in which the same gene is introduced into a wild-type strain.
摘要翻译： 通过对通过基因破坏衍生自枯草芽孢杆菌的菌株的广泛分析，提供具有各种酶的良好生产力的新型枯草芽孢杆菌突变菌株。 根据本发明的枯草芽孢杆菌突变菌株具有通过缺失缺陷区列中列出的区域而制备的基因组结构。 当与将相同基因导入野生型菌株的情况相比，当引入编码这种分泌型靶蛋白的基因以使其能够被表达时，这些枯草芽孢杆菌突变菌株中的每一种都能显着提高蛋白质的分泌生产力 。
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公开(公告)号：US20090221055A1
公开(公告)日：2009-09-03
申请号：US12083539
申请日：2006-09-25
IPC分类号： C12N1/21
CPC分类号： C12N15/75 , C12N9/2417 , C12N9/54 , C12P21/02
摘要： Novel Bacillus subtilis mutant strains having good productivity of various enzymes are provided through extensive analysis of strains that are derived from Bacillus subtilis via gene disruption. The Bacillus subtilis mutant strains according to the present invention have genomic structures prepared by deletion of regions listed in the columns for deficient regions. Each of these Bacillus subtilis mutant strains exerts significantly improved secretory productivity of a protein when a gene encoding such a secretory target protein is introduced so that it can be expressed, compared with a case in which the same gene is introduced into a wild-type strain.
摘要翻译： 通过对通过基因破坏衍生自枯草芽孢杆菌的菌株的广泛分析，提供具有各种酶的良好生产力的新型枯草芽孢杆菌突变菌株。 根据本发明的枯草芽孢杆菌突变菌株具有通过缺失缺陷区列中列出的区域而制备的基因组结构。 当与将相同基因导入野生型菌株的情况相比，当引入编码这种分泌型靶蛋白的基因以使其能够被表达时，这些枯草芽孢杆菌突变菌株中的每一种都能显着提高蛋白质的分泌生产力 。
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公开(公告)号：US07585674B2
公开(公告)日：2009-09-08
申请号：US10479214
申请日：2002-05-28
申请人： Kazuhisa Sawada , Keiji Endo , Tadahiro Ozawa , Masatoshi Tohata , Katsuya Ozaki
发明人： Kazuhisa Sawada , Keiji Endo , Tadahiro Ozawa , Masatoshi Tohata , Katsuya Ozaki
CPC分类号： C07K14/32 , C07K2319/00 , C12N1/20
摘要： A microorganism wherein one or more genes selected from the group of genes participating in sporulation in the middle to late stages of sporulation have been deleted or inactivated; and a process for producing a target product (a protein) by use the microorganism. No spore is formed when this microorganism is employed, thereby enabling production of a target product (a protein) while decreasing energy loss, production of a by-product and specific production speed to decrease unnecessary consumption of a medium. Moreover, the production period can be prolonged, whereby the target product (the protein) can be produced efficiently.
摘要翻译： 一种微生物，其中一个或多个选自参与孢子形成中期至晚期孢子形成的基因的基因已被删除或失活; 以及通过使用微生物生产目标产物（蛋白质）的方法。 当使用该微生物时不形成孢子，从而能够减少能量损失，副产物的产生和特定的生产速度，从而能够生产目标产物（蛋白质），从而减少不必要的培养基消耗。 此外，可以延长生产周期，由此有效地生产目标产物（蛋白质）。
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公开(公告)号：US20080081352A1
公开(公告)日：2008-04-03
申请号：US10578615
申请日：2004-11-05
CPC分类号： C12N9/2437 , C12N15/75 , C12P21/00 , C12Y302/01004
摘要： The present invention provides a recombinant microorganism obtained by transferring, into a host microorganism which is capable of producing protein or polypeptide with increased productivity and which was identified by the present inventors, a gene encoding a protein or polypeptide, and a method for producing a protein or polypeptide by use of the recombinant microorganism. The recombinant microorganism is prepared by transferring, to a mutant strain of microorganism from which any of at least one gene participating in membrane permeation of maltose has been deleted or knocked out, a gene encoding a heterologous protein or polypeptide.
摘要翻译： 本发明提供了一种重组微生物，其通过将能够产生蛋白质或多肽的生物能力提高的宿主微生物转移到本发明人鉴定出的编码蛋白质或多肽的基因以及产生蛋白质的方法中 或多肽。 将重组微生物通过转移到至少一种参与麦芽糖的膜渗透的基因中的任何一种已被缺失或敲除的微生物的突变菌株，编码异源蛋白质或多肽的基因。
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公开(公告)号：US07563611B2
公开(公告)日：2009-07-21
申请号：US10578615
申请日：2004-11-05
IPC分类号： C12N1/20
CPC分类号： C12N9/2437 , C12N15/75 , C12P21/00 , C12Y302/01004
摘要： The present invention provides a recombinant microorganism obtained by transferring, into a host microorganism which is capable of producing protein or polypeptide with increased productivity and which was identified by the present inventors, a gene encoding a protein or polypeptide, and a method for producing a protein or polypeptide by use of the recombinant microorganism.
摘要翻译： 本发明提供了一种重组微生物，其通过将能够产生蛋白质或多肽的生物能力提高的宿主微生物转移到本发明人鉴定出的编码蛋白质或多肽的基因以及产生蛋白质的方法中 或多肽。
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公开(公告)号：US20080009039A1
公开(公告)日：2008-01-10
申请号：US10578613
申请日：2004-11-05
摘要： A recombinant microorganism obtained by transferring, into a host microorganism capable of producing protein or polypeptide with increased productivity, a gene encoding a protein or polypeptide, and a method for producing a protein or polypeptide by use of the recombinant microorganism. The recombinant microorganism is prepared by transferring, to a mutant strain of microorganism from which any of Bacillus subtilis genes comA, yopO, treR, yvbA, cspB, yvaN, yttP, yurK, yozA, licR, sigL, mntR, glcT, yvdE, ykvE, sir, rocR, ccpA, yaaT, yyaA, yycH, yacP, hprK, rsiX, yhdK, and yibO, or one or more genes functionally equivalent to any of these genes have been deleted or knocked out, a gene encoding a heterologous protein or polypeptide.
摘要翻译： 通过将能够产生生产能力的蛋白质或多肽的宿主微生物，编码蛋白质或多肽的基因，以及通过使用重组微生物产生蛋白质或多肽的方法而获得的重组微生物。 通过转移到枯草芽孢杆菌基因comA，yopO，treR，yvbA，cspB，yvaN，yttP，yurK，yozA，licR，sigL，mntR，glcT，yvdE，ykvE中的任一种的微生物突变菌株来制备重组微生物 ，先生，rocR，ccpA，yaaT，yyaA，yycH，yacP，hprK，rsiX，yhdK和yibO，或者与这些基因中的任何一个功能等同的一个或多个基因已被缺失或敲除，编码异源蛋白的基因或 多肽。
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