发明申请
- 专利标题: CHIMERIC GENE CONSTRUCTS FOR GENERATION OF FLUORESCENT TRANSGENIC ORNAMENTAL FISH
- 专利标题(中): 用于生成荧光转基因鱼的基因基因构建体
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申请号: US13334444申请日: 2011-12-22
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公开(公告)号: US20120167241A1公开(公告)日: 2012-06-28
- 发明人: Zhiyuan GONG , Jiangyan HE , Bensheng JU , Toon Jin LAM , Yanfei XU , Tie YAN
- 申请人: Zhiyuan GONG , Jiangyan HE , Bensheng JU , Toon Jin LAM , Yanfei XU , Tie YAN
- 申请人地址: SG Singapore
- 专利权人: NATIONAL UNIVERSITY OF SINGAPORE
- 当前专利权人: NATIONAL UNIVERSITY OF SINGAPORE
- 当前专利权人地址: SG Singapore
- 优先权: SG9900811-2 19990218
- 主分类号: A01K67/027
- IPC分类号: A01K67/027
摘要:
Four zebrafish gene promoters, which are skin specific, muscle specific, skeletal muscle specific and ubiquitously expressed respectively, were isolated and ligated to the 5′ end of the EGFP gene. When the resulting chimeric gene constructs were introduced into zebrafish, the transgenic zebrafish emit green fluorescence under a blue light or ultraviolet light according to the specificity of the promoters used. Thus, new varieties of ornamental fish of different fluorescence patterns, e.g., skin fluorescence, muscle fluorescence, skeletal muscle-specific and/or ubiquitous fluorescence, are developed.
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