公开(公告)号：US20120167241A1

公开(公告)日：2012-06-28

申请号：US13334444

申请日：2011-12-22

申请人： Zhiyuan GONG ,  Jiangyan HE ,  Bensheng JU ,  Toon Jin LAM ,  Yanfei XU ,  Tie YAN

发明人： Zhiyuan GONG ,  Jiangyan HE ,  Bensheng JU ,  Toon Jin LAM ,  Yanfei XU ,  Tie YAN

IPC分类号： A01K67/027

CPC分类号： A01K67/0275 ,  A01K2217/05 ,  A01K2217/20 ,  A01K2227/40 ,  A01K2267/02 ,  A01K2267/0393 ,  C07K14/43595 ,  C12N15/85 ,  C12N15/8509 ,  C12N2830/00 ,  C12N2830/008 ,  C12N2830/42 ,  C12N2830/80 ,  C12N2840/20 ,  G01N33/743

摘要： Four zebrafish gene promoters, which are skin specific, muscle specific, skeletal muscle specific and ubiquitously expressed respectively, were isolated and ligated to the 5′ end of the EGFP gene. When the resulting chimeric gene constructs were introduced into zebrafish, the transgenic zebrafish emit green fluorescence under a blue light or ultraviolet light according to the specificity of the promoters used. Thus, new varieties of ornamental fish of different fluorescence patterns, e.g., skin fluorescence, muscle fluorescence, skeletal muscle-specific and/or ubiquitous fluorescence, are developed.

摘要翻译： 分离出四种斑马鱼基因启动子，分别是皮肤特异性，肌肉特异性，骨骼肌特异性和无处不在表达，并连接到EGFP基因的5'末端。 当所得的嵌合基因构建体被引入斑马鱼中时，根据所用启动子的特异性，转基因斑马鱼在蓝光或紫外光下发射绿色荧光。 因此，开发了不同荧光图案的观赏鱼的新品种，例如皮肤荧光，肌肉荧光，骨骼肌特异性和/或普遍存在的荧光。