公开(公告)号：US08378169B2

公开(公告)日：2013-02-19

申请号：US13334444

申请日：2011-12-22

申请人： Zhiyuan Gong ,  Jiangyan He ,  Bensheng Ju ,  Toong Jin Lam ,  Yanfei Xu ,  Tie Yan

发明人： Zhiyuan Gong ,  Jiangyan He ,  Bensheng Ju ,  Toong Jin Lam ,  Yanfei Xu ,  Tie Yan

IPC分类号： A01K67/027

CPC分类号： A01K67/0275 ,  A01K2217/05 ,  A01K2217/20 ,  A01K2227/40 ,  A01K2267/02 ,  A01K2267/0393 ,  C07K14/43595 ,  C12N15/85 ,  C12N15/8509 ,  C12N2830/00 ,  C12N2830/008 ,  C12N2830/42 ,  C12N2830/80 ,  C12N2840/20 ,  G01N33/743

摘要： Four zebrafish gene promoters, which are skin specific, muscle specific, skeletal muscle specific and ubiquitously expressed respectively, were isolated and ligated to the 5′ end of the EGFP gene. When the resulting chimeric gene constructs were introduced into zebrafish, the transgenic zebrafish emit green fluorescence under a blue light or ultraviolet light according to the specificity of the promoters used. Thus, new varieties of ornamental fish of different fluorescence patterns, e.g., skin fluorescence, muscle fluorescence, skeletal muscle-specific and/or ubiquitous fluorescence, are developed.

摘要翻译： 分离出四种斑马鱼基因启动子，分别是皮肤特异性，肌肉特异性，骨骼肌特异性和无处不在表达，并连接到EGFP基因的5'末端。 当所得的嵌合基因构建体被引入斑马鱼中时，根据所用启动子的特异性，转基因斑马鱼在蓝光或紫外光下发射绿色荧光。 因此，开发了不同荧光图案的观赏鱼的新品种，例如皮肤荧光，肌肉荧光，骨骼肌特异性和/或普遍存在的荧光。

公开(公告)号：US20120167241A1

公开(公告)日：2012-06-28

申请号：US13334444

申请日：2011-12-22

申请人： Zhiyuan GONG ,  Jiangyan HE ,  Bensheng JU ,  Toon Jin LAM ,  Yanfei XU ,  Tie YAN

发明人： Zhiyuan GONG ,  Jiangyan HE ,  Bensheng JU ,  Toon Jin LAM ,  Yanfei XU ,  Tie YAN

IPC分类号： A01K67/027

CPC分类号： A01K67/0275 ,  A01K2217/05 ,  A01K2217/20 ,  A01K2227/40 ,  A01K2267/02 ,  A01K2267/0393 ,  C07K14/43595 ,  C12N15/85 ,  C12N15/8509 ,  C12N2830/00 ,  C12N2830/008 ,  C12N2830/42 ,  C12N2830/80 ,  C12N2840/20 ,  G01N33/743

摘要： Four zebrafish gene promoters, which are skin specific, muscle specific, skeletal muscle specific and ubiquitously expressed respectively, were isolated and ligated to the 5′ end of the EGFP gene. When the resulting chimeric gene constructs were introduced into zebrafish, the transgenic zebrafish emit green fluorescence under a blue light or ultraviolet light according to the specificity of the promoters used. Thus, new varieties of ornamental fish of different fluorescence patterns, e.g., skin fluorescence, muscle fluorescence, skeletal muscle-specific and/or ubiquitous fluorescence, are developed.

摘要翻译： 分离出四种斑马鱼基因启动子，分别是皮肤特异性，肌肉特异性，骨骼肌特异性和无处不在表达，并连接到EGFP基因的5'末端。 当所得的嵌合基因构建体被引入斑马鱼中时，根据所用启动子的特异性，转基因斑马鱼在蓝光或紫外光下发射绿色荧光。 因此，开发了不同荧光图案的观赏鱼的新品种，例如皮肤荧光，肌肉荧光，骨骼肌特异性和/或普遍存在的荧光。

公开(公告)号：US20070071776A1

公开(公告)日：2007-03-29

申请号：US11530169

申请日：2006-09-08

申请人： Yoke SIN ,  Toong LAM ,  Zhiyuan GONG

发明人： Yoke SIN ,  Toong LAM ,  Zhiyuan GONG

IPC分类号： A61K48/00 ,  A61K39/00

CPC分类号： A61K39/002 ,  A61K2039/55516 ,  A61K2039/60 ,  C07K14/44

摘要： A recombinant vaccine that is useful for immunizing fish against ciliated ectoparasitic protozoans that cause white spot disease also known as ‘ich’. The disease is caused by Ichthyophthirius multifiliis which is a ciliated ectorparasitic protozoan that is an obligate parasite and therefore cannot grow in vitro. The instant vaccine replaces chemotherapy and vaccines prepared from I. Multifiliis production in live fish. The effective element of the vaccine is a recombinant fusion protein derived from an artificial DNA sequence for immobilization antigen, repeat I, of Ichthyophthirius multifiliis. The novel DNA nucleotide and amino acid sequences are disclosed.

摘要翻译： 一种重组疫苗，可用于对引起白点病的纤毛性外寄生原生动物进行免疫，也称为“ich”。 这种疾病是由作为一种专性寄生虫的纤毛寄生虫原生动物，因此不能在体外生长的鱼腥草多糖引起的。 即时疫苗取代了从活鱼中的多菌素生产制备的化疗和疫苗。 该疫苗的有效成分是来源于人工DNA序列的重组融合蛋白，用于固定抗原，重复I，Ichthyophthirius multifiliis。 公开了新的DNA核苷酸和氨基酸序列。

公开(公告)号：US07834239B2

公开(公告)日：2010-11-16

申请号：US10605708

申请日：2003-10-21

申请人： Zhiyuan Gong ,  Jiangyan He ,  Bensheng Ju ,  Toong Jin Lam ,  Yanfei Xu ,  Tie Yan

发明人： Zhiyuan Gong ,  Jiangyan He ,  Bensheng Ju ,  Toong Jin Lam ,  Yanfei Xu ,  Tie Yan

IPC分类号： A01K67/027

CPC分类号： A01K67/0275 ,  A01K2217/05 ,  A01K2217/20 ,  A01K2227/40 ,  A01K2267/02 ,  A01K2267/0393 ,  C07K14/43595 ,  C12N15/85 ,  C12N15/8509 ,  C12N2830/00 ,  C12N2830/008 ,  C12N2830/42 ,  C12N2830/80 ,  C12N2840/20 ,  G01N33/743

摘要： Four zebrafish gene promoters, which are skin specific, muscle specific, skeletal muscle specific and ubiquitously expressed respectively, were isolated and ligated to the 5′ end of the EGFP gene. When the resulting chimeric gene constructs were introduced into zebrafish, the transgenic zebrafish emit green fluorescence under a blue light or ultraviolet light according to the specificity of the promoters used. Thus, new varieties of ornamental fish of different fluorescence patterns, e.g., skin fluorescence, muscle fluorescence, skeletal muscle-specific and/or ubiquitous fluorescence, are developed.

摘要翻译： 分离出四种斑马鱼基因启动子，分别是皮肤特异性，肌肉特异性，骨骼肌特异性和无处不在表达，并连接到EGFP基因的5'末端。 当所得的嵌合基因构建体被引入斑马鱼中时，根据所用启动子的特异性，转基因斑马鱼在蓝光或紫外光下发射绿色荧光。 因此，开发了不同荧光图案的观赏鱼的新品种，例如皮肤荧光，肌肉荧光，骨骼肌特异性和/或普遍存在的荧光。

公开(公告)号：US20080052787A1

公开(公告)日：2008-02-28

申请号：US11749032

申请日：2007-05-15

申请人： Zhiyuan Gong ,  Jiangyan He ,  Bensheng Ju ,  Toong Lam ,  Yanfei Xu ,  Tie Yan

发明人： Zhiyuan Gong ,  Jiangyan He ,  Bensheng Ju ,  Toong Lam ,  Yanfei Xu ,  Tie Yan

IPC分类号： A01K67/027

CPC分类号： A01K67/0275 ,  A01K2217/05 ,  A01K2217/20 ,  A01K2227/40 ,  A01K2267/02 ,  A01K2267/0393 ,  C07K14/43595 ,  C12N15/85 ,  C12N15/8509 ,  C12N2830/00 ,  C12N2830/008 ,  C12N2830/42 ,  C12N2830/80 ,  C12N2840/20 ,  G01N33/743

摘要： Four zebrafish gene promoters, which are skin specific, muscle specific, skeletal muscle specific and ubiquitously expressed respectively, were isolated and ligated to the 5′ end of the EGFP gene. When the resulting chimeric gene constructs were introduced into zebrafish, the transgenic zebrafish emit green fluorescence under a blue light or ultraviolet light according to the specificity of the promoters used. Thus, new varieties of ornamental fish of different fluorescence patterns, e.g., skin fluorescence, muscle fluorescence, skeletal muscle-specific and/or ubiquitous fluorescence, are developed.

摘要翻译： 分离出四种斑马鱼基因启动子，分别是皮肤特异性，肌肉特异性，骨骼肌特异性和无处不在表达，并连接到EGFP基因的5'末端。 当所得的嵌合基因构建体被引入斑马鱼中时，根据所用启动子的特异性，转基因斑马鱼在蓝光或紫外光下发射绿色荧光。 因此，开发了不同荧光图案的观赏鱼的新品种，例如皮肤荧光，肌肉荧光，骨骼肌特异性和/或普遍存在的荧光。

公开(公告)号：US07135613B1

公开(公告)日：2006-11-14

申请号：US09913898

申请日：1999-07-16

申请人： Zhiyuan Gong ,  Jiangyan He ,  Bensheng Ju ,  Toong Jin Lam ,  Yanfei Xu ,  Tie Yan

发明人： Zhiyuan Gong ,  Jiangyan He ,  Bensheng Ju ,  Toong Jin Lam ,  Yanfei Xu ,  Tie Yan

IPC分类号： A01K67/00 ,  A01K67/027 ,  C07H21/02 ,  C07H21/04

CPC分类号： A01K67/0275 ,  A01K2217/05 ,  A01K2217/20 ,  A01K2227/40 ,  A01K2267/02 ,  A01K2267/0393 ,  C07K14/43595 ,  C12N15/85 ,  C12N15/8509 ,  C12N2830/00 ,  C12N2830/008 ,  C12N2830/42 ,  C12N2830/80 ,  C12N2840/20 ,  G01N33/743

摘要： Four zebrafish gene promoters, which are skin specific, muscle specific, skeletal muscle specific and ubiquitously expressed, respectively, were isolated and ligated to the 5′ end of the EGFP gene. When the resulting chimeric gene constructs were introduced into zebrafish, the transgenic zebrafish emit green fluorescence under a blue light or ultra-violet light according to the specificity of the promoters used. Thus, new varieties of ornamental fish of different fluorescence patters, e.g., skin fluorescence, muscle fluorescence, skeletal muscle-specific and/or ubiquitous fluorescence, are developed.

摘要翻译： 分离出四种斑马鱼基因启动子，分别是皮肤特异性，肌肉特异性，骨骼肌特异性和普遍表达，并连接到EGFP基因的5'末端。 当所得到的嵌合基因构建体被引入斑马鱼时，根据所用启动子的特异性，转基因斑马鱼在蓝光或紫外光下发射绿色荧光。 因此，开发了不同荧光图案的新品种的观赏鱼，例如皮肤荧光，肌肉荧光，骨骼肌特异性和/或普遍存在的荧光。

公开(公告)号：US06307020B1

公开(公告)日：2001-10-23

申请号：US09117121

申请日：1998-11-20

申请人： Choy Hew ,  Zhiyuan Gong

发明人： Choy Hew ,  Zhiyuan Gong

IPC分类号： C07K100

CPC分类号： C12N15/8273 ,  C07K14/461

摘要： A family of related intracellular skin type antifreeze polypeptides and corresponding coding nucleic acids are provided. These are the first skin type intracellular antifreeze polypeptides and coding nucleic acids ever reported. The polypeptides are naturally expressed in the skin of Winter Flounder, and skin specific promoters are also provided. The polypeptides are used to make cells cold-resistant, and to improve the palatability of cold foods and liquids. Cold resistant eukaryotes and prokaryotes, including plants, animals and bacteria are made using the skin-type intracellular antifreeze polypeptides and nucleic acids.

摘要翻译： 提供了一系列相关的细胞内皮肤型防冻多肽和相应的编码核酸。 这些是曾经报道的第一种皮肤型细胞内抗冻多肽和编码核酸。 多肽在冬季比目鱼的皮肤中天然表达，并提供皮肤特异性启动子。 多肽用于使细胞耐寒，并提高冷食品和液体的适口性。 使用皮肤型细胞内抗冻多肽和核酸制备耐寒真核生物和原核生物，包括植物，动物和细菌。

公开(公告)号：US09763430B2

公开(公告)日：2017-09-19

申请号：US13738704

申请日：2013-01-10

申请人： Zhiyuan Gong ,  Jiangyan He ,  Bensheng Ju ,  Toong Jin Lam ,  Yanfei Xu ,  Tie Yan

发明人： Zhiyuan Gong ,  Jiangyan He ,  Bensheng Ju ,  Toong Jin Lam ,  Yanfei Xu ,  Tie Yan

IPC分类号： A01K67/027 ,  C07K14/435 ,  C12N15/85 ,  G01N33/74

CPC分类号： A01K67/0275 ,  A01K2217/05 ,  A01K2217/20 ,  A01K2227/40 ,  A01K2267/02 ,  A01K2267/0393 ,  C07K14/43595 ,  C12N15/85 ,  C12N15/8509 ,  C12N2830/00 ,  C12N2830/008 ,  C12N2830/42 ,  C12N2830/80 ,  C12N2840/20 ,  G01N33/743

摘要： Four zebrafish gene promoters, which are skin specific, muscle specific, skeletal muscle specific and ubiquitously expressed respectively, were isolated and ligated to the 5′ end of the EGFP gene. When the resulting chimeric gene constructs were introduced into zebrafish, the transgenic zebrafish emit green fluorescence under a blue light or ultraviolet light according to the specificity of the promoters used. Thus, new varieties of ornamental fish of different fluorescence patterns, e.g., skin fluorescence, muscle fluorescence, skeletal muscle-specific and/or ubiquitous fluorescence, are developed.

公开(公告)号：US20140007265A1

公开(公告)日：2014-01-02

申请号：US13738704

申请日：2013-01-10

申请人： Zhiyuan GONG ,  Jiangyan HE ,  Bensheng JU ,  Toong Jin LAM ,  Yanfei XU ,  Tie YAN

发明人： Zhiyuan GONG ,  Jiangyan HE ,  Bensheng JU ,  Toong Jin LAM ,  Yanfei XU ,  Tie YAN

IPC分类号： A01K67/027

CPC分类号： A01K67/0275 ,  A01K2217/05 ,  A01K2217/20 ,  A01K2227/40 ,  A01K2267/02 ,  A01K2267/0393 ,  C07K14/43595 ,  C12N15/85 ,  C12N15/8509 ,  C12N2830/00 ,  C12N2830/008 ,  C12N2830/42 ,  C12N2830/80 ,  C12N2840/20 ,  G01N33/743

摘要： Four zebrafish gene promoters, which are skin specific, muscle specific, skeletal muscle specific and ubiquitously expressed respectively, were isolated and ligated to the 5′ end of the EGFP gene. When the resulting chimeric gene constructs were introduced into zebrafish, the transgenic zebrafish emit green fluorescence under a blue light or ultraviolet light according to the specificity of the promoters used. Thus, new varieties of ornamental fish of different fluorescence patterns, e.g., skin fluorescence, muscle fluorescence, skeletal muscle-specific and/or ubiquitous fluorescence, are developed.

摘要翻译： 分离出四种斑马鱼基因启动子，分别是皮肤特异性，肌肉特异性，骨骼肌特异性和无处不在表达，并连接到EGFP基因的5'末端。 当所得的嵌合基因构建体被引入斑马鱼中时，根据所用启动子的特异性，转基因斑马鱼在蓝光或紫外光下发射绿色荧光。 因此，开发了不同荧光图案的观赏鱼的新品种，例如皮肤荧光，肌肉荧光，骨骼肌特异性和/或普遍存在的荧光。

公开(公告)号：US08153858B2

公开(公告)日：2012-04-10

申请号：US11749032

申请日：2007-05-15

申请人： Zhiyuan Gong ,  Jiangyan He ,  Bensheng Ju ,  Toong Jin Lam ,  Yanfei Xu ,  Tie Yan

发明人： Zhiyuan Gong ,  Jiangyan He ,  Bensheng Ju ,  Toong Jin Lam ,  Yanfei Xu ,  Tie Yan

IPC分类号： A01K67/027

CPC分类号： A01K67/0275 ,  A01K2217/05 ,  A01K2217/20 ,  A01K2227/40 ,  A01K2267/02 ,  A01K2267/0393 ,  C07K14/43595 ,  C12N15/85 ,  C12N15/8509 ,  C12N2830/00 ,  C12N2830/008 ,  C12N2830/42 ,  C12N2830/80 ,  C12N2840/20 ,  G01N33/743

摘要： Four zebrafish gene promoters, which are skin specific, muscle specific, skeletal muscle specific and ubiquitously expressed respectively, were isolated and ligated to the 5′ end of the EGFP gene. When the resulting chimeric gene constructs were introduced into zebrafish, the transgenic zebrafish emit green fluorescence under a blue light or ultraviolet light according to the specificity of the promoters used. Thus, new varieties of ornamental fish of different fluorescence patterns, e.g., skin fluorescence, muscle fluorescence, skeletal muscle-specific and/or ubiquitous fluorescence, are developed.

摘要翻译： 分离出四种斑马鱼基因启动子，分别是皮肤特异性，肌肉特异性，骨骼肌特异性和无处不在表达，并连接到EGFP基因的5'末端。 当所得的嵌合基因构建体被引入斑马鱼中时，根据所用启动子的特异性，转基因斑马鱼在蓝光或紫外光下发射绿色荧光。 因此，开发了不同荧光图案的观赏鱼的新品种，例如皮肤荧光，肌肉荧光，骨骼肌特异性和/或普遍存在的荧光。