Invention Application
- Patent Title: METHODS FOR CONJUGATING NUCLEIC ACIDS WITH SMALL MOLECULES
- Patent Title (中): 用小分子结合核酸的方法
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Application No.: US13188989Application Date: 2011-07-22
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Publication No.: US20120177573A1Publication Date: 2012-07-12
- Inventor: Tzu-Pin Wang , Yi-Jhang Ciou
- Applicant: Tzu-Pin Wang , Yi-Jhang Ciou
- Applicant Address: TW Kaohsiung City
- Assignee: KAOHSIUNG MEDICAL UNIVERSITY
- Current Assignee: KAOHSIUNG MEDICAL UNIVERSITY
- Current Assignee Address: TW Kaohsiung City
- Priority: TW100101162 20110112
- Main IPC: A61K49/00
- IPC: A61K49/00 ; C07H1/00 ; A61K31/7105 ; C07D495/04 ; A61K48/00 ; G01N33/53 ; C07K1/107
Abstract:
A method for conjugating a nucleic acid with a molecule is provided. The method includes steps of (a) reacting the nucleic acid having a 5′-monophosphate with an activating agent in a first buffer to form a solution; (b) mixing an alcohol with the solution formed in the step (a) to obtain an intermediate; and (c) dissolving the intermediate in a second buffer containing an ethylenediaminetetraacetic acid (EDTA) and adding a nucleophile thereinto to react the intermediate with the nucleophile.
Public/Granted literature
- US08765934B2 Methods for conjugating nucleic acids with small molecules Public/Granted day:2014-07-01
Information query
