公开(公告)号：US08765934B2

公开(公告)日：2014-07-01

申请号：US13188989

申请日：2011-07-22

Applicant: Tzu-Pin Wang ,  Yi-Jhang Ciou

Inventor： Tzu-Pin Wang ,  Yi-Jhang Ciou

IPC: C07H21/00 ,  C12Q1/68 ,  C07D293/10 ,  C07D235/02

CPC classification number: C07H21/04 ,  A61K31/7105 ,  C07D495/04 ,  C07H21/02 ,  C07K1/1077

Abstract: A method for conjugating a nucleic acid with a molecule is provided. The method includes steps of (a) reacting the nucleic acid having a 5′-monophosphate with an activating agent in a first buffer to form a solution; (b) mixing an alcohol with the solution formed in the step (a) to obtain an intermediate; and (c) dissolving the intermediate in a second buffer containing an ethylenediaminetetraacetic acid (EDTA) and adding a nucleophile thereinto to react the intermediate with the nucleophile.

Abstract translation: 提供了用于将核酸与分子缀合的方法。 该方法包括以下步骤：（a）使具有5'-单磷酸的核酸与活化剂在第一缓冲液中反应形成溶液; （b）将醇与步骤（a）中形成的溶液混合以获得中间体; 和（c）将中间体溶解在含有乙二胺四乙酸（EDTA）的第二缓冲液中并加入亲核试剂以使中间体与亲核试剂反应。

公开(公告)号：US20120177573A1

公开(公告)日：2012-07-12

申请号：US13188989

申请日：2011-07-22

Applicant: Tzu-Pin Wang ,  Yi-Jhang Ciou

Inventor： Tzu-Pin Wang ,  Yi-Jhang Ciou

IPC: A61K49/00 ,  C07H1/00 ,  A61K31/7105 ,  C07D495/04 ,  A61K48/00 ,  G01N33/53 ,  C07K1/107

CPC classification number: C07H21/04 ,  A61K31/7105 ,  C07D495/04 ,  C07H21/02 ,  C07K1/1077

Abstract: A method for conjugating a nucleic acid with a molecule is provided. The method includes steps of (a) reacting the nucleic acid having a 5′-monophosphate with an activating agent in a first buffer to form a solution; (b) mixing an alcohol with the solution formed in the step (a) to obtain an intermediate; and (c) dissolving the intermediate in a second buffer containing an ethylenediaminetetraacetic acid (EDTA) and adding a nucleophile thereinto to react the intermediate with the nucleophile.

Abstract translation: 提供了用于将核酸与分子缀合的方法。 该方法包括以下步骤：（a）使具有5'-单磷酸的核酸与活化剂在第一缓冲液中反应形成溶液; （b）将醇与步骤（a）中形成的溶液混合以获得中间体; 和（c）将中间体溶解在含有乙二胺四乙酸（EDTA）的第二缓冲液中并加入亲核试剂以使中间体与亲核试剂反应。

公开(公告)号：US20080090689A1

公开(公告)日：2008-04-17

申请号：US11545567

申请日：2006-10-11

Applicant: Tzu-Pin Wang

Inventor： Tzu-Pin Wang

IPC: F16H47/08

CPC classification number: F16D25/061 ,  F16D25/06

Abstract: A clutch consists of transmission gears, pistons, a switch on each piston, and a passive gear; the transmission gears are fitted on a fly wheel; each piston is pivoted to an inner side of a clutch cover at a fixed end, and pivoted to a respective transmission gear at a moving end; the switches are provided for changing valves of the pistons between closed and open states, and have resilience so that they will return to closed position automatically when external force moving them to open position disappears; the passive gear is joined to a clutch shaft and engaged with the transmission gears; when the piston valves are in closed state, the piston moving ends will be immobilized to fix the transmission gears; therefore, the transmission gears will cause rotation of the passive gear and the clutch shaft when the fly wheel is rotating.

Abstract translation: 离合器包括传动齿轮，活塞，每个活塞上的开关和被动齿轮; 传动齿轮安装在飞轮上; 每个活塞在固定端枢转到离合器盖的内侧，并且在移动端枢转到相应的传动齿轮; 提供开关用于在闭合和打开状态之间改变活塞的阀，并且具有弹性，使得当外力将其移动到打开位置时，它们将自动返回到关闭位置; 被动齿轮连接到离合器轴并与传动齿轮接合; 当活塞阀处于关闭状态时，活塞移动端将被固定以固定传动齿轮; 因此，当飞轮旋转时，传动齿轮将引起被动齿轮和离合器轴的旋转。