发明申请
- 专利标题: PRODUCTION OF NON-YEAST STEROLS BY YEAST
- 专利标题(中): YEAST生产非YEAST STEROLS
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申请号: US13510636申请日: 2010-11-24
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公开(公告)号: US20120231495A1公开(公告)日: 2012-09-13
- 发明人: Hans-Peter Hohmann , Martin Lehmann , Muriel Merkamm , Denis Pompon
- 申请人: Hans-Peter Hohmann , Martin Lehmann , Muriel Merkamm , Denis Pompon
- 优先权: EP09014988.1 20091203
- 国际申请: PCT/EP2010/068127 WO 20101124
- 主分类号: C12P33/06
- IPC分类号: C12P33/06 ; C12N15/53 ; C12N15/81 ; C12N1/19
摘要:
This invention relates to the production of 7-dehydrocholesterol, 25-hydroxy-7-dehydrocholesterol, and 25-hydroxy ergosterol in yeast such as Saccharomyces cerevisiae. It also related to various enzymes catalyzing the reduction of the double bond at position 24 of lanosterol, dimethyl zymosterol, methyl zymosterol, zymosterol, cholesta-7, 24-dienol, or cholesta-5,7,24-trienol; or the hydroxylation at position 25 of ergosterol, 7-dehydrocholesterol, cholesta-8-enol, and cholesta-7-enol. It also relates to various nucleic acids encoding cholesterol C25-hydroxylases and sterol Δ24-reductases and their use to produce and hydroxylate 7-dehydrocholesterol or ergosterol. It also relates to the yeast strains so produced, and methods of making these sterols comprising the steps of cultivation a transformed yeast cell, and harvesting the resulting sterol(s).
公开/授权文献
- US09624521B2 Production of non-yeast sterols by yeast 公开/授权日:2017-04-18