公开(公告)号：US09624521B2

公开(公告)日：2017-04-18

申请号：US13510636

申请日：2010-11-24

申请人： Hans-Peter Hohmann ,  Martin Lehmann ,  Muriel Merkamm ,  Denis Pompon

发明人： Hans-Peter Hohmann ,  Martin Lehmann ,  Muriel Merkamm ,  Denis Pompon

IPC分类号： C12N9/02 ,  C12N9/10 ,  C12N1/19 ,  C12P33/06 ,  C12P33/00

CPC分类号： C12P33/00 ,  C12N9/001 ,  C12N9/0067 ,  C12N9/0071 ,  C12N9/0083 ,  C12N9/1007 ,  C12P33/06

摘要： This invention relates to the production of 7-dehydrocholesterol, 25-hydroxy-7-dehydrocholesterol, and 25-hydroxy ergosterol in yeast such as Saccharomyces cerevisiae. It also relates to various enzymes catalyzing the reduction of the double bond at position 24 of lanosterol, dimethyl zymosterol, methyl zymosterol, zymosterol, cholesta-7, 24-dienol, or cholesta-5,7,24-trienol; or the hydroxylation at position 25 of ergosterol, 7-dehydrocholesterol, cholesta-8-enol, and cholesta-7-enol. It also relates to various nucleic acids encoding cholesterol C25-hydroxylases and sterol Δ24-reductases and their use to produce and hydroxylate 7-dehydrocholesterol or ergosterol. It also relates to the yeast strains so produced, and methods of making these sterols that include the steps of cultivaton a transformed yeast cell, and harvesting the resulting sterol(s).

公开(公告)号：US20120231495A1

公开(公告)日：2012-09-13

申请号：US13510636

申请日：2010-11-24

申请人： Hans-Peter Hohmann ,  Martin Lehmann ,  Muriel Merkamm ,  Denis Pompon

发明人： Hans-Peter Hohmann ,  Martin Lehmann ,  Muriel Merkamm ,  Denis Pompon

IPC分类号： C12P33/06 ,  C12N15/53 ,  C12N15/81 ,  C12N1/19

CPC分类号： C12P33/00 ,  C12N9/001 ,  C12N9/0067 ,  C12N9/0071 ,  C12N9/0083 ,  C12N9/1007 ,  C12P33/06

摘要： This invention relates to the production of 7-dehydrocholesterol, 25-hydroxy-7-dehydrocholesterol, and 25-hydroxy ergosterol in yeast such as Saccharomyces cerevisiae. It also related to various enzymes catalyzing the reduction of the double bond at position 24 of lanosterol, dimethyl zymosterol, methyl zymosterol, zymosterol, cholesta-7, 24-dienol, or cholesta-5,7,24-trienol; or the hydroxylation at position 25 of ergosterol, 7-dehydrocholesterol, cholesta-8-enol, and cholesta-7-enol. It also relates to various nucleic acids encoding cholesterol C25-hydroxylases and sterol Δ24-reductases and their use to produce and hydroxylate 7-dehydrocholesterol or ergosterol. It also relates to the yeast strains so produced, and methods of making these sterols comprising the steps of cultivation a transformed yeast cell, and harvesting the resulting sterol(s).

摘要翻译： 本发明涉及酵母如酿酒酵母中7-脱氢胆固醇，25-羟基-7-脱氢胆固醇和25-羟基麦角甾醇的生产。 它还涉及催化羊毛甾醇，二甲基十八烷醇，甲基十八醇，十八酮，胆甾烯-7,24-二烯醇或胆石酯-5,7,24-三烯酚在位置24处双键还原的各种酶。 或麦角甾醇，7-脱氢胆固醇，胆甾烯-8-烯醇和胆甾烯-7-烯醇在25位的羟基化。 它还涉及编码胆固醇C25-羟化酶和甾醇和Dgr; 24-还原酶的各种核酸，以及它们用于产生7-羟基胆固醇或麦角固醇羟基化的用途。 它还涉及如此制备的酵母菌株，以及制备这些甾醇的方法包括培养转化的酵母细胞的步骤，并收获所得的甾醇。

公开(公告)号：US06569650B1

公开(公告)日：2003-05-27

申请号：US09373731

申请日：1999-08-13

申请人： Muriel Merkamm ,  Armel Guyonvarch ,  Achim Marx

发明人： Muriel Merkamm ,  Armel Guyonvarch ,  Achim Marx

IPC分类号： C12P1304

CPC分类号： C12N9/0089 ,  C12P13/08 ,  C12R1/15

摘要： The present invention is directed to nucleotide sequences coding for the superoxide dismutase (sod) gene from Corynebacterium melassecola. It includes processes for the fermentative preparation of nucleotides, vitamins and L-amino acids using coryneform bacteria in which the sod gene is amplified.

摘要翻译： 本发明涉及编码来自黑曲霉（Corynebacterium melassecola）的超氧化物歧化酶（sod）基因的核苷酸序列。 它包括使用其中sod基因被扩增的棒状细菌发酵制备核苷酸，维生素和L-氨基酸的方法。