发明申请
US20120252081A1 USING CELL DEBRIS GENERATED FROM PHA RECOVERY FOR ENHANCED CELL GROWTH AND BIOPOLYESTER FORMATION
审中-公开
使用从PHA恢复产生的细胞凋亡增强细胞生长和生物聚合物形成
- 专利标题: USING CELL DEBRIS GENERATED FROM PHA RECOVERY FOR ENHANCED CELL GROWTH AND BIOPOLYESTER FORMATION
- 专利标题(中): 使用从PHA恢复产生的细胞凋亡增强细胞生长和生物聚合物形成
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申请号: US13502323申请日: 2009-10-16
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公开(公告)号: US20120252081A1公开(公告)日: 2012-10-04
- 发明人: Jian Yu
- 申请人: Jian Yu
- 申请人地址: IT San Giorgio Di Piano (Bologna)
- 专利权人: BIO ON S.R.L.
- 当前专利权人: BIO ON S.R.L.
- 当前专利权人地址: IT San Giorgio Di Piano (Bologna)
- 国际申请: PCT/IB2009/007142 WO 20091016
- 主分类号: C12P7/62
- IPC分类号: C12P7/62
摘要:
The present invention relates to a process for producing biodegradable polymeric materials including polyhydroxyalkanoates (PHAs) by using the cell debris left from PHA recovery and purification. The process comprises: (a) cultivating PHA-producing microbial cells in a medium solution containing an organic carbon source to form PHAs that are accumulated in the cells as inclusion bodies; (b) harvesting the cells from the spent medium and solubilizing the non-PHA cell mass to obtain a PHA solid and a cell debris solution; (c) separating the PHA solid from the cell debris solution; (d) feeding the cell debris solution to the cultivation step (a). By reusing the cell debris generated from PHA recovery, the invention avoids disposal of a large amount of aqueous waste. In addition, a remarkable increase of cell growth and PHA synthesis is achieved, because the cell debris can be readily assimilated by the microbial cells as the nutrients.