Provided is a method of generating and detecting a preamble that may significantly increase accuracy of frame synchronization while avoiding a low frequency domain having great noise power and minimizing hardware complexity and power consumption in a communication system of a digital direct transmission scheme applicable to human body communication. A method of generating a preamble according to an exemplary embodiment of the present disclosure includes: generating a first pseudo noise code and a second pseudo noise code that are different from each other; generating a plurality of same first sub preambles by line-coding the first pseudo noise code; and generating a second sub preamble behind the plurality of first sub preambles by line-coding the second pseudo noise code.