Disclosed is a cell culture vessel of the present invention, which is composed of a space enclosed by a housing that supports a medium and a cell attachment part, disposed on the bottom surface of the space, that attaches and supports the cells, the cell attachment part having a cell immobilizing mechanism that guides the cells in the space to the cell attachment part to immobilize there and a cell detachment mechanism that detaches the cells attached in the cell attachment part, the cell immobilizing mechanism having a step of applying a voltage to an electrode disposed in the cell attachment part to generate a heterogeneous electric field in the space, and the cell detachment mechanism having a step of applying a voltage to an electrode disposed in the cell attachment part to induce electrolyte in the space.