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1.发明申请Biological Sample Packaging Container and Biological Sample Conveyance Method Using Same 审中-公开生物样品包装容器和使用其的生物样品输送方法公开(公告)号：US20150231628A1
公开(公告)日：2015-08-20
申请号：US14426201
申请日：2012-09-11
Applicant: Takayuki Nozaki , Masaharu Kiyama , Guangbin Zhou , Shizu Matsuoka , Ryota Nakajima , Naoko Senda
Inventor： Takayuki Nozaki , Masaharu Kiyama , Guangbin Zhou , Shizu Matsuoka , Ryota Nakajima , Naoko Senda
CPC classification number: B01L3/508 , A01N1/0242 , B01L2200/18 , B01L2300/04 , B65B3/04 , C12M45/22
Abstract: The present invention provides a biological sample packaging container including: a sample storage container having a recessed part; a lid member that seals the upper surface of the sample storage container; and at least one gasket that is provided on the rear surface of the lid member or on the upper surface of the sample storage container, wherein: the sample storage container has a function of holding a biological sample therein; the lid member includes a first lid member provided with flow channel tubes that allow liquid and gas to be moved between the sample storage container and the outside, and a second lid member having no flow channel tubes; and the sample storage container is configured in such a manner that the first lid member and the second lid member can be replaced by each other.
Abstract translation: 本发明提供一种生物样品包装容器，其包括：具有凹部的样品储存容器; 封闭所述样本收容容器的上表面的盖部件; 以及至少一个垫片，其设置在所述盖部件的后表面或所述样本收容容器的上表面上，其中：所述样本收纳容器具有将生物体样品保持在其中的功能; 盖构件包括：第一盖构件，其设置有允许液体和气体在样品储存容器和外部之间移动的流动通道管和不具有流动通道管的第二盖构件; 并且样本收容容器构成为能够使第一盖部件和第二盖部件相互替换。
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公开(公告)号：US20140186941A1
公开(公告)日：2014-07-03
申请号：US14124754
申请日：2012-06-05
Applicant: Guangbin Zhou , Taizo Miyazaki , Ryota Nakajima , Toyoshige Kobayashi , Shizu Matsuoka
Inventor： Guangbin Zhou , Taizo Miyazaki , Ryota Nakajima , Toyoshige Kobayashi , Shizu Matsuoka
IPC: C12M1/00
CPC classification number: C12M47/04 , C12M23/20 , C12M25/08 , C12M33/00 , C12M35/02 , C12M41/36 , C12M47/02
Abstract: Disclosed is a cell culture vessel of the present invention, which is composed of a space enclosed by a housing that supports a medium and a cell attachment part, disposed on the bottom surface of the space, that attaches and supports the cells, the cell attachment part having a cell immobilizing mechanism that guides the cells in the space to the cell attachment part to immobilize there and a cell detachment mechanism that detaches the cells attached in the cell attachment part, the cell immobilizing mechanism having a step of applying a voltage to an electrode disposed in the cell attachment part to generate a heterogeneous electric field in the space, and the cell detachment mechanism having a step of applying a voltage to an electrode disposed in the cell attachment part to induce electrolyte in the space.
Abstract translation: 公开了本发明的细胞培养容器，其由由支撑培养基的壳体包围的空间和设置在空间的底面上的细胞附着部分构成，其附着并支撑细胞，细胞附着物 具有细胞固定机构的部分，其将空间中的细胞引导到细胞附着部分以固定在其上，以及细胞分离机构，其分离附着在细胞附着部分中的细胞，细胞固定机构具有向 电极，其设置在电池安装部中，以在该空间中产生异质电场，并且电池拆卸机构具有向设置在电池安装部中的电极施加电压以在该空间中诱导电解质的步骤。
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公开(公告)号：US20140087455A1
公开(公告)日：2014-03-27
申请号：US14111060
申请日：2012-04-04
Applicant: Toyoshige Kobayashi , Ryota Nakajima , Takayuki Nozaki , Shizu Matsuoka
Inventor： Toyoshige Kobayashi , Ryota Nakajima , Takayuki Nozaki , Shizu Matsuoka
IPC: C12M1/00
Abstract: The present invention provides a carrier device including a culture vessel and a flow channel, which have a closed structure, for supplying cell liquid or a culture medium into the flow channel; and a method of aseptically injecting liquid, such as the cell or the culture medium, into the culture vessel and the flow channel. The present invention provides a carrier jig 19 including a drive base 27, a culture vessel base 21 that holds a culture vessel 20, a culture medium base 32 that holds a cell bag 60 and the like for the culture medium and the cell, and a detachable flow channel 40, those of which are held by a lift 98 to be carried to a cell culture device. A part of the flow channel 40 and the cell bag 60 mounted on the carrier jig 19 are put into a clean space 78. The cell and the culture medium are supplied into the flow channel 40 by using the cell bag 60 and other bags in the clean space, and then, an injection port 89 is closed. The respective bases and the flow channel are simultaneously mounted to the cell culture device by using the carrier jig 19. Thus, the aseptic cell culture can be realized.
Abstract translation: 本发明提供了一种载体装置，其包括具有封闭结构的培养容器和流动通道，用于将细胞液或培养基供给到流路中; 以及将诸如细胞或培养基的液体无菌注入培养容器和流路的方法。 本发明提供一种载体夹具19，其包括驱动基座27，保持培养容器20的培养容器基座21，保持培养基和细胞的细胞袋60等的培养基底32，以及 可拆卸的流动通道40，其中的流动通道由电梯98保持以运送到细胞培养装置。 将流路40的一部分和安装在载体夹具19上的电池袋60放入清洁空间78中。电池和培养基通过使用电池袋60和其他袋子供应到流路40中 清洁空间，然后关闭注入口89。 通过使用载体夹具19将各基底和流路同时安装在细胞培养装置上。因此，可以实现无菌细胞培养。
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公开(公告)号：US09487747B2
公开(公告)日：2016-11-08
申请号：US14124754
申请日：2012-06-05
Applicant: Guangbin Zhou , Taizo Miyazaki , Ryota Nakajima , Toyoshige Kobayashi , Shizu Matsuoka
Inventor： Guangbin Zhou , Taizo Miyazaki , Ryota Nakajima , Toyoshige Kobayashi , Shizu Matsuoka
CPC classification number: C12M47/04 , C12M23/20 , C12M25/08 , C12M33/00 , C12M35/02 , C12M41/36 , C12M47/02
Abstract: Disclosed is a cell culture vessel of the present invention, which is composed of a space enclosed by a housing that supports a medium and a cell attachment part, disposed on the bottom surface of the space, that attaches and supports the cells, the cell attachment part having a cell immobilizing mechanism that guides the cells in the space to the cell attachment part to immobilize there and a cell detachment mechanism that detaches the cells attached in the cell attachment part, the cell immobilizing mechanism having a step of applying a voltage to an electrode disposed in the cell attachment part to generate a heterogeneous electric field in the space, and the cell detachment mechanism having a step of applying a voltage to an electrode disposed in the cell attachment part to induce electrolyte in the space.
Abstract translation: 公开了本发明的细胞培养容器，其由由支撑培养基的壳体包围的空间和设置在空间的底面上的细胞附着部分构成，其附着并支撑细胞，细胞附着物 具有细胞固定机构的部分，其将空间中的细胞引导到细胞附着部分以固定在其上，以及细胞分离机构，其分离附着在细胞附着部分中的细胞，细胞固定机构具有向 电极，其设置在电池安装部中，以在该空间中产生异质电场，并且电池拆卸机构具有向设置在电池安装部中的电极施加电压以在该空间中诱导电解质的步骤。
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公开(公告)号：US20140141499A1
公开(公告)日：2014-05-22
申请号：US14129122
申请日：2012-06-25
Applicant: Ryota Nakajima , Shizu Matsuoka , Toyoshige Kobayashi
Inventor： Ryota Nakajima , Shizu Matsuoka , Toyoshige Kobayashi
IPC: C12M1/34
Abstract: Provided is a cell culture device having a culture area for culturing the stem or progenitor cells, from which tissues are formed. The cell culture device includes an oxygen adjusting part that adjusts the oxygen supply in the culture area, a controller that controls the oxygen adjusting part, and another controller that controls a first oxygen supply during a first period of the period, in which the stem cells and the progenitor cells differentiate; and a second oxygen supply greater in amount than the first supply during a second period, during which the stem cells and the progenitor cells differentiate, out of the whole culture period, and that controls the oxygen adjusting part based on the growth progress of the stem cells and the progenitor cells through self-replication to change the first oxygen supply to the second oxygen supply.
Abstract translation: 本发明提供一种细胞培养装置，其具有用于培养形成组织的茎或祖细胞的培养区域。 细胞培养装置包括调节培养区域中的氧供给的氧调节部，控制氧调节部的控制器，以及控制在第一期间的第一氧供给的控制器，其中，干细胞 祖细胞分化; 以及在整个培养期间干细胞和祖细胞分化的第二时期期间比第一次供应量大的第二供氧量，并且基于茎的生长进程来控制氧调节部分 细胞和祖细胞通过自我复制将第一氧气供应改变为第二氧气供应。
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Patent Agency Ranking
公开(公告)号：US09951305B2
公开(公告)日：2018-04-24
申请号：US14111060
申请日：2012-04-04
Applicant: Toyoshige Kobayashi , Ryota Nakajima , Takayuki Nozaki , Shizu Matsuoka
Inventor： Toyoshige Kobayashi , Ryota Nakajima , Takayuki Nozaki , Shizu Matsuoka
Abstract: The present invention provides a carrier device including a culture vessel and a flow channel, which have a closed structure, for supplying cell liquid or a culture medium into the flow channel; and a method of aseptically injecting liquid, such as the cell or the culture medium, into the culture vessel and the flow channel. The present invention provides a carrier jig 19 including a drive base 27, a culture vessel base 21 that holds a culture vessel 20, a culture medium base 32 that holds a cell bag 60 and the like for the culture medium and the cell, and a detachable flow channel 40, those of which are held by a lift 98 to be carried to a cell culture device. A part of the flow channel 40 and the cell bag 60 mounted on the carrier jig 19 are put into a clean space 78. The cell and the culture medium are supplied into the flow channel 40 by using the cell bag 60 and other bags in the clean space, and then, an injection port 89 is closed. The respective bases and the flow channel are simultaneously mounted to the cell culture device by using the carrier jig 19. Thus, the aseptic cell culture can be realized.
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公开(公告)号：US20150072401A1
公开(公告)日：2015-03-12
申请号：US14381659
申请日：2012-03-29
Applicant: Takayuki Nozaki , Guangbin Zhou , Ryota Nakajima , Shizu Matsuoka , Toyoshige Kobayashi , Naoko Senda
Inventor： Takayuki Nozaki , Guangbin Zhou , Ryota Nakajima , Shizu Matsuoka , Toyoshige Kobayashi , Naoko Senda
Abstract: In a closed system culture vessel to be used in an automated culture apparatus, some of the ports perform both liquid supply and gas supply functions. Culture medium is thereby made to flow always in one direction. The culture vessel is also configured so that, in the complete culture medium exchange process and the effluent recovery process for analyzing culture medium components, waste liquid medium does not get mixed into fresh culture medium.
Abstract translation: 在用于自动培养装置的封闭系统培养容器中，一些端口同时执行液体供应和气体供应功能。 由此使培养基始终沿一个方向流动。 培养容器也配置成使得在完全培养基的交换过程和用于分析培养基成分的废水回收过程中，废液介质不会混入新鲜培养基中。
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公开(公告)号：US08883492B2
公开(公告)日：2014-11-11
申请号：US13331237
申请日：2011-12-20
Applicant: Toyoshige Kobayashi , Ryota Nakajima , Takayuki Nozaki , Shizu Matsuoka
Inventor： Toyoshige Kobayashi , Ryota Nakajima , Takayuki Nozaki , Shizu Matsuoka
CPC classification number: C12M41/14
Abstract: A cell culture apparatus has four sections, including a cell culture chamber, refrigerator, control unit, and intermediate chamber. The cell culture chamber includes a culture vessel base as a holding member for holding and installing a culture vessel, a microscope for photographing a cell image within the culture vessel, and a drive base which has drive mechanisms such as a pump and a valve to be connected to a flow channel having a closed structure including a tank. With the drive base within the cell culture chamber as the center, the rotatable culture vessel is arranged in the horizontal direction and the refrigerator in which the culture medium base containing the cell fluid and culture medium is installed is arranged in the vertical direction. The arrangement configuration is space-saving and provides a short distance between the instruments.
Abstract translation: 细胞培养装置具有四个部分，包括细胞培养室，冰箱，控制单元和中间室。 细胞培养室包括培养容器底座作为用于保持和安装培养容器的保持构件，用于拍摄培养容器内的细胞图像的显微镜和具有诸如泵和阀的驱动机构的驱动基座 连接到具有包括罐的封闭结构的流动通道。 以细胞培养室内的驱动基座为中心，沿水平方向布置可旋转的培养容器，并且在垂直方向上布置有包含细胞液和培养基的培养基的冰箱。 排列配置节省空间并且在仪器之间提供短距离。
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9.发明授权Composition for electrochromic devices and electrochromic display device using the same 有权用于电致变色装置的组合物和使用其的电致变色显示装置公开(公告)号：US08462419B2
公开(公告)日：2013-06-11
申请号：US13283242
申请日：2011-10-27
Applicant: Katsuyoshi Hoshino , Yosuke Oikawa , Mai Ando , Ryota Nakajima , Katsuyuki Murashiro , Toshiki Komatsu
Inventor： Katsuyoshi Hoshino , Yosuke Oikawa , Mai Ando , Ryota Nakajima , Katsuyuki Murashiro , Toshiki Komatsu
CPC classification number: C09K9/02 , G02F1/1506 , G02F2202/04
Abstract: There is provided a composition for electrochromic devices which clearly present only two states, a white-colored state and a decolored state. The composition for electrochromic devices contains (A) a specific quaternary ammonium salt, (B) a supporting electrolyte containing bromide ion, and (C) a solvent containing water as an essential component, and (A) is soluble in (C).
Abstract translation: 提供了一种用于电致变色装置的组合物，其清楚地仅呈现出两种状态，即白色状态和脱色状态。 用于电致变色装置的组合物含有（A）特定的季铵盐，（B）含有溴离子的支持电解质，（C）以水为主要成分的溶剂，（A）可溶于（C）。
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公开(公告)号：US20130115690A1
公开(公告)日：2013-05-09
申请号：US13809094
申请日：2011-07-07
Applicant: Ryota Nakajima , Noboru Moriya , Toyoshige Kobayashi , Shizu Matsuoka , Takayuki Nozaki , Keisuke Mori
Inventor： Ryota Nakajima , Noboru Moriya , Toyoshige Kobayashi , Shizu Matsuoka , Takayuki Nozaki , Keisuke Mori
IPC: C12M1/12
Abstract: Disclosed is a cell culture vessel that can prevent liquid leakage from the cell culture space and entry of particles containing microorganisms from outside the cell culture space, and that can prepare regenerated tissue in a manner that is safe and results in peace of mind. The cell culture vessel is mounted to a cell culture device, holds/cultures cells, and is characterized by being provided with: a culture solution holding section that holds a culture solution, a protruding structure section that is for supplying and discharging the aforementioned culture solution; and a culture solution duct that passes from the aforementioned protruding structure section to the aforementioned culture solution holding section.
Abstract translation: 公开了一种细胞培养容器，其可以防止液体从细胞培养空间泄漏并从细胞培养空间外进入含有微生物的颗粒，并且可以以安全的方式制备再生组织，并导致安心。 将细胞培养容器安装在细胞培养装置上，保存/培养细胞，其特征在于具有：保持培养液的培养液保持部，用于向上述培养液供给和排出的突出结构部 ; 以及从上述突出结构部向上述培养液保持部通过的培养液导管。
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