-
公开(公告)号：US10407657B2
公开(公告)日：2019-09-10
申请号：US14425331
申请日：2012-09-05
申请人： Guangbin Zhou , Takayuki Nozaki , Shizu Matsuoka , Ryota Nakajima , Masaharu Kiyama , Kenichi Minami
发明人： Guangbin Zhou , Takayuki Nozaki , Shizu Matsuoka , Ryota Nakajima , Masaharu Kiyama , Kenichi Minami
摘要： The purpose of the present invention is to provide a valve mechanism which features a simple structure adapted to collectively control the supply of gas or culture medium to a plurality of cultivation containers for simultaneous cultivation and to enhance the reliability of the valve mechanism. The valve mechanism of the invention is a valve mechanism for opening and closing passages for liquid or gas. The valve mechanism includes: clip members adapted to close a plurality of tube passages, respectively; a multiple cam member for opening the clip members in sequence by making contact with the clip members; and a rotational mechanism for rotating the multiple cam member. The clip member includes: a spring for closing a clip thereof; and a clip contact portion for a cam contact portion of the multiple cam member to make contact therewith.
-
2.发明申请CELL CULTURING VESSEL, AND CELL CULTURING METHOD AND AUTOMATED CELL CULTURING DEVICE USING SAME 审中-公开细胞培养血管和细胞培养方法和使用相同的自动细胞培养装置公开(公告)号：US20150175949A1
公开(公告)日：2015-06-25
申请号：US14408670
申请日：2012-06-19
申请人： Guangbin Zhou , Takayuki Nozaki , Shizu Matsuoka
发明人： Guangbin Zhou , Takayuki Nozaki , Shizu Matsuoka
摘要： The purpose of the present invention is to decrease cell culturing vessel running cost and reduce the amount of industrial waste. A cell culturing vessel provided with a container frame and a bottom face support wherein: the cell culturing space is formed by the frame and the bottom face support; the frame is provided with a securing means for securing the bottom face support so as to be attachable and detachable; and an exchangeable culturing surface is provided on the bottom face support.
摘要翻译： 本发明的目的是减少细胞培养容器的运行成本并减少工业废物的量。 一种细胞培养容器，其具有容器框架和底面支撑体，其中：所述细胞培养空间由所述框架和所述底面支撑体形成; 框架设置有用于固定底面支撑件以固定和拆卸的固定装置; 并且在底面支撑件上设置可交换的培养面。
-
3.发明申请Biological Sample Packaging Container and Biological Sample Conveyance Method Using Same 审中-公开生物样品包装容器和使用其的生物样品输送方法公开(公告)号：US20150231628A1
公开(公告)日：2015-08-20
申请号：US14426201
申请日：2012-09-11
申请人： Takayuki Nozaki , Masaharu Kiyama , Guangbin Zhou , Shizu Matsuoka , Ryota Nakajima , Naoko Senda
发明人： Takayuki Nozaki , Masaharu Kiyama , Guangbin Zhou , Shizu Matsuoka , Ryota Nakajima , Naoko Senda
CPC分类号： B01L3/508 , A01N1/0242 , B01L2200/18 , B01L2300/04 , B65B3/04 , C12M45/22
摘要： The present invention provides a biological sample packaging container including: a sample storage container having a recessed part; a lid member that seals the upper surface of the sample storage container; and at least one gasket that is provided on the rear surface of the lid member or on the upper surface of the sample storage container, wherein: the sample storage container has a function of holding a biological sample therein; the lid member includes a first lid member provided with flow channel tubes that allow liquid and gas to be moved between the sample storage container and the outside, and a second lid member having no flow channel tubes; and the sample storage container is configured in such a manner that the first lid member and the second lid member can be replaced by each other.
摘要翻译： 本发明提供一种生物样品包装容器，其包括：具有凹部的样品储存容器; 封闭所述样本收容容器的上表面的盖部件; 以及至少一个垫片，其设置在所述盖部件的后表面或所述样本收容容器的上表面上，其中：所述样本收纳容器具有将生物体样品保持在其中的功能; 盖构件包括：第一盖构件，其设置有允许液体和气体在样品储存容器和外部之间移动的流动通道管和不具有流动通道管的第二盖构件; 并且样本收容容器构成为能够使第一盖部件和第二盖部件相互替换。
-
公开(公告)号：US20140186941A1
公开(公告)日：2014-07-03
申请号：US14124754
申请日：2012-06-05
IPC分类号： C12M1/00
摘要： Disclosed is a cell culture vessel of the present invention, which is composed of a space enclosed by a housing that supports a medium and a cell attachment part, disposed on the bottom surface of the space, that attaches and supports the cells, the cell attachment part having a cell immobilizing mechanism that guides the cells in the space to the cell attachment part to immobilize there and a cell detachment mechanism that detaches the cells attached in the cell attachment part, the cell immobilizing mechanism having a step of applying a voltage to an electrode disposed in the cell attachment part to generate a heterogeneous electric field in the space, and the cell detachment mechanism having a step of applying a voltage to an electrode disposed in the cell attachment part to induce electrolyte in the space.
摘要翻译： 公开了本发明的细胞培养容器，其由由支撑培养基的壳体包围的空间和设置在空间的底面上的细胞附着部分构成，其附着并支撑细胞，细胞附着物 具有细胞固定机构的部分，其将空间中的细胞引导到细胞附着部分以固定在其上，以及细胞分离机构，其分离附着在细胞附着部分中的细胞，细胞固定机构具有向 电极，其设置在电池安装部中，以在该空间中产生异质电场，并且电池拆卸机构具有向设置在电池安装部中的电极施加电压以在该空间中诱导电解质的步骤。
-
公开(公告)号：US20140087455A1
公开(公告)日：2014-03-27
申请号：US14111060
申请日：2012-04-04
IPC分类号： C12M1/00
摘要： The present invention provides a carrier device including a culture vessel and a flow channel, which have a closed structure, for supplying cell liquid or a culture medium into the flow channel; and a method of aseptically injecting liquid, such as the cell or the culture medium, into the culture vessel and the flow channel. The present invention provides a carrier jig 19 including a drive base 27, a culture vessel base 21 that holds a culture vessel 20, a culture medium base 32 that holds a cell bag 60 and the like for the culture medium and the cell, and a detachable flow channel 40, those of which are held by a lift 98 to be carried to a cell culture device. A part of the flow channel 40 and the cell bag 60 mounted on the carrier jig 19 are put into a clean space 78. The cell and the culture medium are supplied into the flow channel 40 by using the cell bag 60 and other bags in the clean space, and then, an injection port 89 is closed. The respective bases and the flow channel are simultaneously mounted to the cell culture device by using the carrier jig 19. Thus, the aseptic cell culture can be realized.
摘要翻译： 本发明提供了一种载体装置，其包括具有封闭结构的培养容器和流动通道，用于将细胞液或培养基供给到流路中; 以及将诸如细胞或培养基的液体无菌注入培养容器和流路的方法。 本发明提供一种载体夹具19，其包括驱动基座27，保持培养容器20的培养容器基座21，保持培养基和细胞的细胞袋60等的培养基底32，以及 可拆卸的流动通道40，其中的流动通道由电梯98保持以运送到细胞培养装置。 将流路40的一部分和安装在载体夹具19上的电池袋60放入清洁空间78中。电池和培养基通过使用电池袋60和其他袋子供应到流路40中 清洁空间，然后关闭注入口89。 通过使用载体夹具19将各基底和流路同时安装在细胞培养装置上。因此，可以实现无菌细胞培养。
-
公开(公告)号：US20140045252A1
公开(公告)日：2014-02-13
申请号：US14113857
申请日：2012-04-02
IPC分类号： C12M1/42
摘要： Provided is a cell cultivation container equipped with electrodes, which enables automatic culturing, observation of automatically cultured cells, and measurement of electrical resistances. A circular electrode (10) is arranged on a base section or a side surface of a frame body (2) of a cell cultivation container, in such a manner that allows observation of cells. Additionally, a rod-shaped electrode (11) is arranged on a lid section (3) of the cell cultivation container, in such a manner that allows observation of cells. In an alternative configuration, the cell cultivation container has a flow channel (8) made of an electrically conductive material. An alternating current generator (13) is connected between the electrode (10) and the electrode (11), and the transepithelial electrical resistances of cells are measured during culturing. This configuration enables automatic culturing, observation of automatically cultured cells, and measurement of electrical resistances.
摘要翻译： 本发明提供一种装有电极的细胞培养容器，其能够自动培养，观察自动培养的细胞，并测量电阻。 在细胞培养容器的框体（2）的基部或侧面上配置圆形电极（10），可以观察细胞。 另外，在细胞培养容器的盖部（3）上配置棒状电极（11），以能够观察细胞。 在替代配置中，细胞培养容器具有由导电材料制成的流动通道（8）。 在电极（10）和电极（11）之间连接有交流发生器（13），并且在培养期间测量细胞的跨上皮电阻。 该配置能够自动培养，观察自动培养的细胞和测量电阻。
-
公开(公告)号：US20130143307A1
公开(公告)日：2013-06-06
申请号：US13816069
申请日：2010-08-12
IPC分类号： C12M1/36
CPC分类号： C12M1/36 , C12M23/14 , C12M23/42 , C12M27/10 , C12M27/20 , C12M29/10 , C12M29/20 , C12M41/00
摘要： In order to uniformly sow cells on the culture surface of a cartridge-type closed culture vessel and remove bubbles contaminating in liquid culture medium in the course of automatic culture, an automatic culture equipment is provided with a flow-controlling mechanism section for solving the non-uniformity in cell distribution by, after filling up a culture space in the cartridge-type closed culture vessel, said cartridge-type closed culture vessel being in an upright position, with cell suspension, turning the cartridge-type closed culture vessel into a horizontal position, and then repeatedly supplying the cell suspension and sucking the same multiple times to thereby create a mixing flow in the cell suspension. In this process, the liquid in channels is efficiently sent by applying a reduced pressure and an elevated pressure respectively to a channel on one side and a channel on the opposite side, using two syringes and check valves connected to the channels, to thereby load forces to the liquid from both sides. The liquid can be sent without any cell loss by conducting the same operations of the flow-controlling mechanism section in a tank and in a cell bag.
摘要翻译： 为了在筒式封闭培养容器的培养表面上均匀地播种细胞，并且在自动培养过程中去除在液体培养基中污染的气泡，自动培养装置设有流动控制机构部分， - 细胞分布均匀性，在填充盒式封闭培养容器中的培养空间后，所述盒式封闭培养容器处于直立位置，具有细胞悬浮液，将盒式封闭培养容器转换成水平 位置，然后反复供给细胞悬浮液并多次吸吮，从而在细胞悬液中产生混合流。 在这个过程中，通过使用两个注射器和连接到通道的止回阀，将减压和升高的压力分别施加到一侧的通道和相对侧上的通道上来有效地发送通道中的液体，从而加载力 来自双方的液体。 通过进行槽内和流通池中的流动控制机构部分的相同操作，可以不发生任何细胞损失而送出液体。
-
公开(公告)号：US20120164721A1
公开(公告)日：2012-06-28
申请号：US13331237
申请日：2011-12-20
IPC分类号： C12M3/02
CPC分类号： C12M41/14
摘要： A cell culture apparatus has four sections, including a cell culture chamber, refrigerator, control unit, and intermediate chamber. The cell culture chamber includes a culture vessel base as a holding member for holding and installing a culture vessel, a microscope for photographing a cell image within the culture vessel, and a drive base which has drive mechanisms such as a pump and a valve to be connected to a flow channel having a closed structure including a tank. With the drive base within the cell culture chamber as the center, the rotatable culture vessel is arranged in the horizontal direction and the refrigerator in which the culture medium base containing the cell fluid and culture medium is installed is arranged in the vertical direction. The arrangement configuration is space-saving and provides a short distance between the instruments.
摘要翻译： 细胞培养装置具有四个部分，包括细胞培养室，冰箱，控制单元和中间室。 细胞培养室包括培养容器底座作为用于保持和安装培养容器的保持构件，用于拍摄培养容器内的细胞图像的显微镜和具有诸如泵和阀的驱动机构的驱动基座 连接到具有包括罐的封闭结构的流动通道。 以细胞培养室内的驱动基座为中心，沿水平方向布置可旋转的培养容器，并且在垂直方向上布置有包含细胞液和培养基的培养基的冰箱。 排列配置节省空间并且在仪器之间提供短距离。
-
公开(公告)号：US09487747B2
公开(公告)日：2016-11-08
申请号：US14124754
申请日：2012-06-05
摘要： Disclosed is a cell culture vessel of the present invention, which is composed of a space enclosed by a housing that supports a medium and a cell attachment part, disposed on the bottom surface of the space, that attaches and supports the cells, the cell attachment part having a cell immobilizing mechanism that guides the cells in the space to the cell attachment part to immobilize there and a cell detachment mechanism that detaches the cells attached in the cell attachment part, the cell immobilizing mechanism having a step of applying a voltage to an electrode disposed in the cell attachment part to generate a heterogeneous electric field in the space, and the cell detachment mechanism having a step of applying a voltage to an electrode disposed in the cell attachment part to induce electrolyte in the space.
摘要翻译： 公开了本发明的细胞培养容器，其由由支撑培养基的壳体包围的空间和设置在空间的底面上的细胞附着部分构成，其附着并支撑细胞，细胞附着物 具有细胞固定机构的部分，其将空间中的细胞引导到细胞附着部分以固定在其上，以及细胞分离机构，其分离附着在细胞附着部分中的细胞，细胞固定机构具有向 电极，其设置在电池安装部中，以在该空间中产生异质电场，并且电池拆卸机构具有向设置在电池安装部中的电极施加电压以在该空间中诱导电解质的步骤。
-
10.发明申请CELL CULTURE CONTAINER, AND AUTOMATED CELL SUBCULTURE DEVICE AND CELL SUBCULTURE METHOD USING SAME 审中-公开细胞培养容器和自动细胞培养装置和细胞再培养方法公开(公告)号：US20140302597A1
公开(公告)日：2014-10-09
申请号：US14365315
申请日：2011-12-14
申请人： Guangbin Zhou , Shizu Matsuoka
发明人： Guangbin Zhou , Shizu Matsuoka
IPC分类号： C12N5/071
CPC分类号： C12N5/0602 , C12M23/04 , C12M23/34
摘要： Provided is a cell culture container that makes it possible to subculture cells without a dispensing operation using a pipette and also to prevent microbial contamination in the culture container during cell subculture. The cell culture container is provided, within a culture container (1) forming a sealable first culture zone, with a partition member (4) having a wall surface formed lower than the wall surface of the culture container and forming a second culture zone; and has means for raising and lowering the partition member while the culture container is in a sealed state. Magnets (4A, 5A, 6A) are used as the means for raising and lowering the partition member.
摘要翻译： 提供了一种细胞培养容器，其可以使用移液管不经分注操作传代培养细胞，并且还可以在细胞传代培养期间防止培养容器中的微生物污染。 在形成可密封的第一培养区的培养容器（1）内设置有分隔构件（4），所述分隔构件（4）的壁面形成为低于培养容器的壁面，形成第二培养区域; 并且具有用于在培养容器处于密封状态时升高和降低分隔构件的装置。 磁体（4A，5A，6A）用作升降分隔构件的装置。
-
-
-
-
-
-
-
-
-
搜索结果
15 条记录 (耗时 0.011 秒)
