Disclosed is a device and method for contacting a biological substrate. A non-thermal plasma device delivers a non-thermal plasma discharge using a dielectric conduit, an igniter electrode and a RF electrode. The dielectric conduit fluidicly communicates a gas therethrough and an igniter electrode ionizes at least a portion of the gas. The RF electrode, disposed circumferentially proximate to the exterior of the dielectric conduit, generates non-thermal plasma from the ionized gas. The non-thermal plasma is discharged from the dielectric conduit and contacts a biological substrate. The non-thermal plasma discharge may be suitable for tissue bonding and sterilization applications.