Invention Application
US20150218208A1 METHOD FOR PURIFYING ANTIBODY BY TEMPERATURE-RESPONSIVE CHROMATOGRAPHY
审中-公开
通过温度响应色谱法纯化抗体的方法
- Patent Title: METHOD FOR PURIFYING ANTIBODY BY TEMPERATURE-RESPONSIVE CHROMATOGRAPHY
- Patent Title (中): 通过温度响应色谱法纯化抗体的方法
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Application No.: US14423568Application Date: 2013-08-27
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Publication No.: US20150218208A1Publication Date: 2015-08-06
- Inventor: Ichiro Koguma , Rumiko Ishikawa , Hiroshi Takemasa , Kazuo Okuyama
- Applicant: ASAHI KASEI MEDICAL CO., LTD.
- Applicant Address: JP Tokyo
- Assignee: ASAHI KASEI MEDICAL CO., LTD.
- Current Assignee: ASAHI KASEI MEDICAL CO., LTD.
- Current Assignee Address: JP Tokyo
- Priority: JP2012-186930 20120827; JP2013-086121 20130416
- International Application: PCT/JP2013/072821 WO 20130827
- Main IPC: C07K1/22
- IPC: C07K1/22

Abstract:
There is provided a method for purifying an antibody using temperature-responsive Protein A, wherein different buffer solutions are used in a washing step of washing a stationary phase having the temperature-responsive Protein A and in an elution step of eluting the antibody captured by the stationary phase, the method including the washing step of washing the stationary phase using a buffer solution at a temperature at which the temperature-responsive Protein A and the antibody are bound and having a first salt concentration, and the elution step of eluting the antibody captured by the stationary phase using a buffer solution at a temperature at which the antibody is released from the temperature-responsive Protein A and having a second salt concentration lower than the first salt concentration.
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