Invention Application
US20150218208A1 METHOD FOR PURIFYING ANTIBODY BY TEMPERATURE-RESPONSIVE CHROMATOGRAPHY 审中-公开
通过温度响应色谱法纯化抗体的方法

METHOD FOR PURIFYING ANTIBODY BY TEMPERATURE-RESPONSIVE CHROMATOGRAPHY
Abstract:
There is provided a method for purifying an antibody using temperature-responsive Protein A, wherein different buffer solutions are used in a washing step of washing a stationary phase having the temperature-responsive Protein A and in an elution step of eluting the antibody captured by the stationary phase, the method including the washing step of washing the stationary phase using a buffer solution at a temperature at which the temperature-responsive Protein A and the antibody are bound and having a first salt concentration, and the elution step of eluting the antibody captured by the stationary phase using a buffer solution at a temperature at which the antibody is released from the temperature-responsive Protein A and having a second salt concentration lower than the first salt concentration.
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