公开(公告)号：US20150218208A1

公开(公告)日：2015-08-06

申请号：US14423568

申请日：2013-08-27

Applicant: ASAHI KASEI MEDICAL CO., LTD.

Inventor： Ichiro Koguma ,  Rumiko Ishikawa ,  Hiroshi Takemasa ,  Kazuo Okuyama

IPC: C07K1/22

CPC classification number: C07K1/22 ,  B01D15/3809 ,  B01D15/3876 ,  G01N2030/8813 ,  B01D15/362

Abstract: There is provided a method for purifying an antibody using temperature-responsive Protein A, wherein different buffer solutions are used in a washing step of washing a stationary phase having the temperature-responsive Protein A and in an elution step of eluting the antibody captured by the stationary phase, the method including the washing step of washing the stationary phase using a buffer solution at a temperature at which the temperature-responsive Protein A and the antibody are bound and having a first salt concentration, and the elution step of eluting the antibody captured by the stationary phase using a buffer solution at a temperature at which the antibody is released from the temperature-responsive Protein A and having a second salt concentration lower than the first salt concentration.

Abstract translation: 提供了使用温度敏感性蛋白A纯化抗体的方法，其中在洗涤具有温度敏感性蛋白A的固定相的洗涤步骤中使用不同的缓冲溶液，并且在洗脱步骤中洗脱由 所述方法包括在温度响应性蛋白A和抗体结合并具有第一盐浓度的温度下使用缓冲溶液洗涤固定相的洗涤步骤，以及洗脱被捕获的抗体的洗脱步骤 通过使用缓冲溶液在抗体从温度敏感性蛋白A释放并具有低于第一盐浓度的第二盐浓度的温度下通过固定相。