发明申请
US20150259710A1 DECARBOXYLASE PROTEINS WITH HIGH KETO-ISOVALERATE DECARBOXYLASE ACTIVITY
审中-公开
具有高KETO-ISOVALERATE脱羧酶活性的脱羧蛋白
- 专利标题： DECARBOXYLASE PROTEINS WITH HIGH KETO-ISOVALERATE DECARBOXYLASE ACTIVITY
- 专利标题（中）： 具有高KETO-ISOVALERATE脱羧酶活性的脱羧蛋白
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申请号： US13561560申请日： 2012-07-30
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公开(公告)号： US20150259710A1公开(公告)日： 2015-09-17
- 发明人: Catherine Asleson Dundon , Kevin Roberg-Perez , Christopher Snow , Peter Meinhold
- 申请人： Catherine Asleson Dundon , Kevin Roberg-Perez , Christopher Snow , Peter Meinhold
- 申请人地址： US CO Englewood
- 专利权人： Gevo, Inc.
- 当前专利权人： Gevo, Inc.
- 当前专利权人地址： US CO Englewood
- 主分类号： C12P7/16
- IPC分类号： C12P7/16 ; C12N9/04 ; C12N9/88
摘要：
The present invention relates to recombinant microorganisms comprising an isobutanol producing metabolic pathway and methods of using said recombinant microorganisms to produce isobutanol. In various aspects of the invention, the recombinant microorganisms may comprise at least one nucleic acid molecule encoding a polypeptide with keto-isovalerate decarboxylase (KIVD) activity, wherein said polypeptide is at least about 65%, 70%, 75%, 80%, 85%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, or 99% identical to a polypeptide selected from SEQ ID NOs: 1-214. Also provided are modified decarboxylases exhibiting an improved ability to utilize α-ketoisovalerate as a substrate in various beneficial enzymatic conversions.
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