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    DECARBOXYLASE PROTEINS WITH HIGH KETO-ISOVALERATE DECARBOXYLASE ACTIVITY
    1.
    发明申请
    DECARBOXYLASE PROTEINS WITH HIGH KETO-ISOVALERATE DECARBOXYLASE ACTIVITY 审中-公开
    具有高KETO-ISOVALERATE脱羧酶活性的脱羧蛋白

    公开(公告)号：US20150259710A1

    公开(公告)日：2015-09-17

    申请号：US13561560

    申请日：2012-07-30

    申请人： Catherine Asleson Dundon ,  Kevin Roberg-Perez ,  Christopher Snow ,  Peter Meinhold

    发明人： Catherine Asleson Dundon ,  Kevin Roberg-Perez ,  Christopher Snow ,  Peter Meinhold

    IPC分类号： C12P7/16 ,  C12N9/04 ,  C12N9/88

    CPC分类号： C12P7/16 ,  C12N9/0006 ,  C12N9/88 ,  C12Y101/01001 ,  C12Y101/01086 ,  C12Y401/01 ,  Y02E50/10

    摘要： The present invention relates to recombinant microorganisms comprising an isobutanol producing metabolic pathway and methods of using said recombinant microorganisms to produce isobutanol. In various aspects of the invention, the recombinant microorganisms may comprise at least one nucleic acid molecule encoding a polypeptide with keto-isovalerate decarboxylase (KIVD) activity, wherein said polypeptide is at least about 65%, 70%, 75%, 80%, 85%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, or 99% identical to a polypeptide selected from SEQ ID NOs: 1-214. Also provided are modified decarboxylases exhibiting an improved ability to utilize α-ketoisovalerate as a substrate in various beneficial enzymatic conversions.

    摘要翻译： 本发明涉及包含产生异丁醇的代谢途径的重组微生物和使用所述重组微生物产生异丁醇的方法。 在本发明的各个方面，重组微生物可包含编码具有酮 - 异戊酸脱羧酶（KIVD）活性的多肽的至少一种核酸分子，其中所述多肽至少约65％，70％，75％，80％ 与选自SEQ ID NO：1-214的多肽的85％，90％，91％，92％，93％，94％，95％，96％，97％，98％或99％相同。 还提供了改进的脱羧酶，其表现出在各种有益的酶转化中利用α-酮异戊酸作为底物的改进能力。

    Engineered Microorganisms for the Production of One or More Target Compounds
    5.
    发明申请
    Engineered Microorganisms for the Production of One or More Target Compounds 审中-公开
    用于生产一种或多种靶化合物的工程微生物

    公开(公告)号：US20120045809A1

    公开(公告)日：2012-02-23

    申请号：US13141648

    申请日：2009-12-23

    申请人： Thomas Buelter ,  Peter Meinhold ,  Christopher Smith ,  Aristos Aristidou ,  Catherine Asleson Dundon ,  Jun Urano

    发明人： Thomas Buelter ,  Peter Meinhold ,  Christopher Smith ,  Aristos Aristidou ,  Catherine Asleson Dundon ,  Jun Urano

    IPC分类号： C12P7/16 ,  C12N1/19

    CPC分类号： C12P7/16 ,  C12N15/52 ,  C12N15/81 ,  Y02E50/10

    摘要： The present invention provides recombinant microorganisms comprising an isobutanol producing metabolic pathway and methods of using said recombinant microorganisms to produce isobutanol. In various aspects of the invention, the recombinant microorganisms comprise isobutanol producing metabolic pathway with one or more isobutanol pathway enzymes localized in the mitochondria. In various embodiments described herein, the recombinant microorganisms may be Crabtree-negative yeast microorganisms, microorganisms of the Saccharomyces clade, Crabtree-positive yeast microorganisms, post-WGD (whole genome duplication) yeast microorganisms, pre-WGD (whole genome duplication) yeast microorganisms, and non-fermenting yeast microorganisms.

    摘要翻译： 本发明提供包含产生异丁醇的代谢途径的重组微生物和使用所述重组微生物产生异丁醇的方法。 在本发明的各个方面，重组微生物包含产生异丁醇的代谢途径与一种或多种位于线粒体中的异丁醇途径酶。 在本文所述的各种实施方案中，重组微生物可以是Crabtree阴性酵母微生物，酵母进化枝的微生物，Crabtree阳性酵母微生物，WGD（全基因组复制）酵母微生物，WGD前（全基因组复制）酵母微生物 ，和非发酵酵母微生物。

    YEAST ORGANISM PRODUCING ISOBUTANOL AT A HIGH YIELD
    6.
    发明申请
    YEAST ORGANISM PRODUCING ISOBUTANOL AT A HIGH YIELD 审中-公开
    YEAST ORGANISM在高产量下生产ISOBUTANOL

    公开(公告)号：US20110318799A1

    公开(公告)日：2011-12-29

    申请号：US13229438

    申请日：2011-09-09

    申请人： Reid M. Renny Feldman ,  Uvini Gunawardena ,  Jun Urano ,  Peter Meinhold ,  Aristos A. Aristidou ,  Catherine Asleson Dundon ,  Christopher Smith

    发明人： Reid M. Renny Feldman ,  Uvini Gunawardena ,  Jun Urano ,  Peter Meinhold ,  Aristos A. Aristidou ,  Catherine Asleson Dundon ,  Christopher Smith

    IPC分类号： C12P7/16

    CPC分类号： C12N9/88 ,  C12P7/16 ,  C12Y401/01001 ,  Y02E50/10

    摘要： There is disclosed a method of producing isobutanol. In an embodiment, the method includes providing a microorganism transformed with an isobutanol producing pathway containing at least one exogenous gene. The microorganism is selected to produce isobutanol from a carbon source at a yield of at least 10 percent theoretical. The method includes cultivating the microorganism in a culture medium containing a feedstock providing the carbon source, until isobutanol is produced. The method includes recovering the isobutanol. In one embodiment, the microorganism is a yeast with a Crabtree-negative phenotype. In another embodiment, the microorganism is a yeast microorganism with a Crabtree-positive phenotype. There is disclosed a microorganism for producing isobutanol. In an embodiment, the microorganism includes an isobutanol producing pathway containing at least one exogenous gene, and is selected to produce a recoverable quantity of isobutanol from a carbon source at a yield of at least 10 percent theoretical.

    摘要翻译： 公开了异丁醇的制造方法。 在一个实施方案中，该方法包括提供转化了含有至少一种外源基因的产生异丁醇的途径的微生物。 选择微生物以产生至少10％的理论值从碳源产生异丁醇。 该方法包括在含有提供碳源的原料的培养基中培养微生物，直到产生异丁醇。 该方法包括回收异丁醇。 在一个实施方案中，微生物是具有Crabtree-阴性表型的酵母。 在另一个实施方案中，微生物是具有Crabtree阳性表型的酵母微生物。 公开了用于生产异丁醇的微生物。 在一个实施方案中，微生物包括含有至少一种外源基因的异丁醇生产途径，并且选择其以至少10％理论值的产率从碳源产生可回收量的异丁醇。