The present invention relates to a flexible display technical filed, and particularly to a flexible substrate and a manufacturing method thereof, and a display apparatus comprising the flexible substrate. The flexible substrate comprises a base, a plurality of flexible layers are provided on the base, and a fiber layer is provided between two adjacent flexible layers. The flexible substrate of the present invention is formed by flexible layers and fiber layers, which can effectively prevent the flexible layers from deformation due to a high temperature, can reduce stress and increase adhesion of a thin film on the substrate to the greatest extent, and bending and compression strength of the substrate are improved.