Invention Application
- Patent Title: Genetic Transformation of Bifidobacteria
- Patent Title (中): 双歧杆菌的遗传转化
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Application No.: US15101078Application Date: 2013-12-02
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Publication No.: US20160304885A1Publication Date: 2016-10-20
- Inventor: Douwe van Sinderen , Mary O'Connell Motherway , Debbie Watson , Tamara Smokvina , Peggy Garault
- Applicant: CAMPAGNIE GERVAIS DANONE , UNIVERSITY COLLEGE CORK - NATIONAL UNIVERSITY OF IRELAND, CORK
- Applicant Address: FR Paris IE Cork
- Assignee: Compagnie Gervais Danone,University College Cork - National University of Ireland, Cork
- Current Assignee: Compagnie Gervais Danone,University College Cork - National University of Ireland, Cork
- Current Assignee Address: FR Paris IE Cork
- International Application: PCT/IB2013/002951 WO 20131202
- Main IPC: C12N15/74
- IPC: C12N15/74 ; C12N1/20 ; C12N9/10
Abstract:
The present invention concerns a method for genetically transforming a Bifidobacterium strain comprising a step of methylation of a shuttle vector in an E. coli or a Gram-positive bacterium strain by two type II DNA methyltransferases from a Bifidobacterium: a methyltransferase enzyme that methylates the adenine base at position 4 of the nucleotide sequence RTCAGG and a methyltransferase enzyme that methylates the cytosine base at position 4 of the nucleotide sequence GGWCC. The present invention also concerns genetic tools and culture media useful for carrying out said method.
Public/Granted literature
- US10184127B2 Genetic transformation of bifidobacteria Public/Granted day:2019-01-22
Information query
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