发明申请
- 专利标题： CHEMOENZYMATIC GLYCOENGINEERING OF ANTIBODIES AND FC FRAGMENTS THEREOF
- 专利标题（中）： 抗体及其FC片段的化学成分分析
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申请号： US15256854申请日： 2016-09-06
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公开(公告)号： US20170058040A1公开(公告)日： 2017-03-02
- 发明人: LAI-XI WANG , WEI HUANG
- 申请人： UNIVERSITY OF MARYLAND, BALTIMORE
- 主分类号： C07K16/28
- IPC分类号： C07K16/28 ; A61K47/48 ; C12N9/24 ; C12N1/20 ; C12P21/00
摘要：
The present invention provides for recombinant Endo-S mutants that exhibit reduced hydrolysis activity and increased transglycosylation activity for the synthesis of glycoproteins wherein a desired sialylated oxazoline or synthetic oligosaccharide oxazoline is added to a core fucosylated or nonfucosylated GlcNAc-protein acceptor. Such recombinant Endo-S mutants are useful for efficient glycosylation remodeling of IgG1-Fc domain to provide different antibody glycoforms carrying structurally well-defined Fc N-glycans.
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