- 专利标题: AMPLIFICATION REPORTER WITH BASE-PAIRING OLIGOMERS
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申请号: US15411482申请日: 2017-01-20
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公开(公告)号: US20170166957A1公开(公告)日: 2017-06-15
- 发明人: John F. Regan , Dianna Maar , Dawne N. Shelton , Tina C. Legler , Samantha Cooper , Wei Yang , Eli A. Hefner , Niels Klitgord
- 申请人: Bio-Rad Laboratories, Inc.
- 主分类号: C12Q1/68
- IPC分类号: C12Q1/68
摘要:
System, including methods, apparatus, and compositions, for performing amplification assays with an amplification reporter including a first oligomer and a second oligomer capable of base-pairing with one another below a melting temperature of the reporter. The reporter may have a detectable photoluminescence that is affected, such as reduced, by base-pairing of the first and second oligomers with one another. A target, such as a nucleic acid target sequence, may be amplified in at least one volume, such as a plurality of partitions, above the melting temperature, and photoluminescence of the reporter may be detected from the at least one volume below the melting temperature. A property of the target, such as a concentration of the target, may be determined based on the photoluminescence detected.
公开/授权文献
- US10604789B2 Amplification reporter with base-pairing oligomers 公开/授权日:2020-03-31
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