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    Amplification reporter with base-pairing oligomers
    6.
    发明授权
    Amplification reporter with base-pairing oligomers 有权

    公开(公告)号：US11085070B2

    公开(公告)日：2021-08-10

    申请号：US16834854

    申请日：2020-03-30

    Applicant: Bio-Rad Laboratories, Inc.

    Inventor： John F. Regan ,  Dianna Maar ,  Dawne N. Shelton ,  Tina C. Legler ,  Samantha Cooper ,  Wei Yang ,  Eli A. Hefner ,  Niels Klitgord

    IPC: C12Q1/68 ,  C12Q1/6818 ,  C12Q1/6823 ,  C12Q1/6848

    Abstract: System, including methods, apparatus, and compositions, for performing amplification assays with an amplification reporter including a first oligomer and a second oligomer capable of base-pairing with one another below a melting temperature of the reporter. The reporter may have a detectable photoluminescence that is affected, such as reduced, by base-pairing of the first and second oligomers with one another. A target, such as a nucleic acid target sequence, may be amplified in at least one volume, such as a plurality of partitions, above the melting temperature, and photoluminescence of the reporter may be detected from the at least one volume below the melting temperature. A property of the target, such as a concentration of the target, may be determined based on the photoluminescence detected.

    DIGITAL AMPLIFICATION ASSAYS FOR GENETIC INSTABILITY
    7.
    发明申请
    DIGITAL AMPLIFICATION ASSAYS FOR GENETIC INSTABILITY 审中-公开

    公开(公告)号：US20180363041A1

    公开(公告)日：2018-12-20

    申请号：US16013845

    申请日：2018-06-20

    Applicant: Bio-Rad Laboratories, Inc.

    Inventor： Dianna Maar

    IPC: C12Q1/6848 ,  C12Q1/6832

    Abstract: Methods and compositions for detecting genetic instability using digital amplification assays. The methods may be performed in a set of isolated volumes and generally may involve competitive hybridization of a competitor and a probe/primer with a normal allele and one or more mutant alleles of a microsatellite locus. The competitor may be configured to compete similarly with, or to outcompete, the primer/probe for hybridization with the normal allele. The primer/probe may be configured to outcompete the competitor for hybridization with various mutant alleles of the locus that alter the length of the repetitive sequence by different amounts. Isolated volumes in which the primer/probe outcompetes the competitor may be enumerated, and represent one or more of the mutant alleles. The methods may enable diagnosing microsatellite instability and treating a subject based on the diagnosis.

    Digital amplification assays for genetic instability
    9.
    发明授权
    Digital amplification assays for genetic instability 有权

    公开(公告)号：US11104938B2

    公开(公告)日：2021-08-31

    申请号：US16013845

    申请日：2018-06-20

    Applicant: Bio-Rad Laboratories, Inc.

    Inventor： Dianna Maar

    IPC: C12Q1/68 ,  C12Q1/6848 ,  C12Q1/6832 ,  C12Q1/6858

    Abstract: Methods and compositions for detecting genetic instability using digital amplification assays. The methods may be performed in a set of isolated volumes and generally may involve competitive hybridization of a competitor and a probe/primer with a normal allele and one or more mutant alleles of a microsatellite locus. The competitor may be configured to compete similarly with, or to outcompete, the primer/probe for hybridization with the normal allele. The primer/probe may be configured to outcompete the competitor for hybridization with various mutant alleles of the locus that alter the length of the repetitive sequence by different amounts. Isolated volumes in which the primer/probe outcompetes the competitor may be enumerated, and represent one or more of the mutant alleles. The methods may enable diagnosing microsatellite instability and treating a subject based on the diagnosis.

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