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公开(公告)号：US12024737B2
公开(公告)日：2024-07-02
申请号：US17396148
申请日：2021-08-06
发明人： John F. Regan , Dianna Maar , Dawne N. Shelton , Tina C. Legler , Samantha Cooper , Wei Yang , Eli A. Hefner , Niels Klitgord
IPC分类号： C12Q1/68 , C12Q1/6818 , C12Q1/6823 , C12Q1/6848
CPC分类号： C12Q1/6818 , C12Q1/6823 , C12Q1/6848 , C12Q1/6818 , C12Q2527/107 , C12Q2565/1015 , C12Q1/6823 , C12Q2537/1373 , C12Q2545/107 , C12Q2565/101
摘要： Method of analysis for alleles of a target. In the method, a plurality of fluid volumes may be formed. Each fluid volume may contain a first primer pair to amplify a first allele of a target, a second primer pair to amplify a second allele of the target, a first reporter including a first photoluminophore and providing a primer of the first primer pair, and a second reporter including a second photoluminophore and providing a primer of the second primer pair. Each of the first and second reporters may include an oligomer having a quencher, and the oligomer may be configured to base-pair with the primer of the first primer pair and the primer of the second primer pair. The first and second alleles may be amplified using the first and second primer pairs. Photoluminescence may be detected. A level of each allele may be determined.
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公开(公告)号：US11085070B2
公开(公告)日：2021-08-10
申请号：US16834854
申请日：2020-03-30
发明人： John F. Regan , Dianna Maar , Dawne N. Shelton , Tina C. Legler , Samantha Cooper , Wei Yang , Eli A. Hefner , Niels Klitgord
IPC分类号： C12Q1/68 , C12Q1/6818 , C12Q1/6823 , C12Q1/6848
摘要： System, including methods, apparatus, and compositions, for performing amplification assays with an amplification reporter including a first oligomer and a second oligomer capable of base-pairing with one another below a melting temperature of the reporter. The reporter may have a detectable photoluminescence that is affected, such as reduced, by base-pairing of the first and second oligomers with one another. A target, such as a nucleic acid target sequence, may be amplified in at least one volume, such as a plurality of partitions, above the melting temperature, and photoluminescence of the reporter may be detected from the at least one volume below the melting temperature. A property of the target, such as a concentration of the target, may be determined based on the photoluminescence detected.
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3.发明申请公开(公告)号：US20140162266A1
公开(公告)日：2014-06-12
申请号：US14097923
申请日：2013-12-05
发明人： Niels Klitgord , John Regan , Svilen Tzonev , Eli Hefner , Jennifer R. Berman
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6851 , C12Q2537/16 , C12Q2563/159
摘要： This disclosure provides methods for measuring the copy number for highly amplified and/or abundant genomic loci. Recognized herein is a need for methods for determining nucleic acid copy number, particularly in instances where one locus to be quantified (i.e., the target) is relatively more abundant than a locus of known abundance (i.e., the reference). In some cases, the method involves combining a query nucleic acid sample with a diluting nucleic acid sample and measuring the relative copy number of a target sequence compared with a reference sequence in the combined sample.
摘要翻译： 本公开提供了用于测量高度扩增和/或丰富的基因组基因座的拷贝数的方法。 本文认识到需要用于确定核酸拷贝数的方法，特别是在一个待定量的位点（即目标）相对于已知丰度的基因座（即参考文献）相对更丰富的情况下。 在一些情况下，该方法包括将查询核酸样品与稀释的核酸样品组合，并测量与组合样品中的参考序列相比的靶序列的相对拷贝数。
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公开(公告)号：US10604789B2
公开(公告)日：2020-03-31
申请号：US15411482
申请日：2017-01-20
发明人： John F. Regan , Dianna Maar , Dawne N. Shelton , Tina C. Legler , Samantha Cooper , Wei Yang , Eli A. Hefner , Niels Klitgord
IPC分类号： C12Q1/68 , C12Q1/6818 , C12Q1/6823 , C12Q1/6848
摘要： System, including methods, apparatus, and compositions, for performing amplification assays with an amplification reporter including a first oligomer and a second oligomer capable of base-pairing with one another below a melting temperature of the reporter. The reporter may have a detectable photoluminescence that is affected, such as reduced, by base-pairing of the first and second oligomers with one another. A target, such as a nucleic acid target sequence, may be amplified in at least one volume, such as a plurality of partitions, above the melting temperature, and photoluminescence of the reporter may be detected from the at least one volume below the melting temperature. A property of the target, such as a concentration of the target, may be determined based on the photoluminescence detected.
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公开(公告)号：US09856525B2
公开(公告)日：2018-01-02
申请号：US14217357
申请日：2014-03-17
发明人： Adam M. McCoy , Niels Klitgord
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6851 , C12Q1/6844 , C12Q2537/165 , C12Q2563/159 , C12Q2537/143
摘要： System, including methods, apparatus, and compositions, for performing a digital assay with associated targets. In some embodiments, the assay of associated targets in partitions may lower the limit of detection (LOD) or otherwise increase assay sensitivity, accuracy, and/or specificity. The targets may represent spaced or overlapping regions of the same template and/or each may represent a region from a different complementary strand of the same template. In some embodiments, the associated targets may be provided by a type of biological particle, and the target content of partitions may be used to identify and quantify the type of biological particle in a sample.
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公开(公告)号：US20170166957A1
公开(公告)日：2017-06-15
申请号：US15411482
申请日：2017-01-20
发明人： John F. Regan , Dianna Maar , Dawne N. Shelton , Tina C. Legler , Samantha Cooper , Wei Yang , Eli A. Hefner , Niels Klitgord
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6818 , C12Q1/6823 , C12Q1/6848 , C12Q2527/107 , C12Q2565/1015 , C12Q2537/1373 , C12Q2545/107 , C12Q2565/101
摘要： System, including methods, apparatus, and compositions, for performing amplification assays with an amplification reporter including a first oligomer and a second oligomer capable of base-pairing with one another below a melting temperature of the reporter. The reporter may have a detectable photoluminescence that is affected, such as reduced, by base-pairing of the first and second oligomers with one another. A target, such as a nucleic acid target sequence, may be amplified in at least one volume, such as a plurality of partitions, above the melting temperature, and photoluminescence of the reporter may be detected from the at least one volume below the melting temperature. A property of the target, such as a concentration of the target, may be determined based on the photoluminescence detected.
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公开(公告)号：US09556475B2
公开(公告)日：2017-01-31
申请号：US14457863
申请日：2014-08-12
发明人： John F. Regan , Dianna Maar , Dawne N. Shelton , Tina C. Legler , Samantha Cooper , Wei Yang , Eli A. Hefner , Niels Klitgord
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6818 , C12Q1/6823 , C12Q1/6848 , C12Q2527/107 , C12Q2565/1015 , C12Q2537/1373 , C12Q2545/107 , C12Q2565/101
摘要： System, including methods, apparatus, and compositions, for performing amplification assays with an amplification reporter including a first oligomer and a second oligomer capable of base-pairing with one another below a melting temperature of the reporter. The reporter may have a detectable photoluminescence that is affected, such as reduced, by base-pairing of the first and second oligomers with one another. A target, such as a nucleic acid target sequence, may be amplified in at least one volume, such as a plurality of partitions, above the melting temperature, and photoluminescence of the reporter may be detected from the at least one volume below the melting temperature. A property of the target, such as a concentration of the target, may be determined based on the photoluminescence detected.
摘要翻译： 用于使用扩增报道子进行扩增测定的系统，包括方法，装置和组合物，所述扩增报告基团包括能够在报道分子的熔点以下彼此碱基配对的第一低聚物和第二低聚物。 报告物可能具有受到影响的可检测的光致发光，例如通过第一和第二寡聚体彼此的碱基配对而减少。 可以在高于熔解温度的至少一个体积（例如多个分区）中扩增靶，例如核酸靶序列，并且可以从低于熔解温度的至少一个体积检测报告物的光致发光 。 可以基于检测到的光致发光来确定靶的特性，例如靶的浓度。
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公开(公告)号：US20150148250A1
公开(公告)日：2015-05-28
申请号：US14457863
申请日：2014-08-12
发明人： John F. Regan , Dianna Maar , Dawne N. Shelton , Tina C. Legler , Samantha Cooper , Wei Yang , Eli A. Hefner , Niels Klitgord
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6818 , C12Q1/6823 , C12Q1/6848 , C12Q2527/107 , C12Q2565/1015 , C12Q2537/1373 , C12Q2545/107 , C12Q2565/101
摘要： System, including methods, apparatus, and compositions, for performing amplification assays with an amplification reporter including a first oligomer and a second oligomer capable of base-pairing with one another below a melting temperature of the reporter. The reporter may have a detectable photoluminescence that is affected, such as reduced, by base-pairing of the first and second oligomers with one another. A target, such as a nucleic acid target sequence, may be amplified in at least one volume, such as a plurality of partitions, above the melting temperature, and photoluminescence of the reporter may be detected from the at least one volume below the melting temperature. A property of the target, such as a concentration of the target, may be determined based on the photoluminescence detected.
摘要翻译： 用于使用扩增报道子进行扩增测定的系统，包括方法，装置和组合物，所述扩增报告基团包括能够在报道分子的熔点以下彼此碱基配对的第一低聚物和第二低聚物。 报告物可能具有受到影响的可检测的光致发光，例如通过第一和第二寡聚体彼此的碱基配对而减少。 可以在高于熔解温度的至少一个体积（例如多个分区）中扩增靶，例如核酸靶序列，并且可以从低于熔解温度的至少一个体积检测报告物的光致发光 。 可以基于检测到的光致发光来确定靶的特性，例如靶的浓度。
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公开(公告)号：US20140274786A1
公开(公告)日：2014-09-18
申请号：US14217357
申请日：2014-03-17
发明人： Adam M. McCoy , Niels Klitgord
CPC分类号： C12Q1/6851 , C12Q1/6844 , C12Q2537/165 , C12Q2563/159 , C12Q2537/143
摘要： System, including methods, apparatus, and compositions, for performing a digital assay with associated targets. In some embodiments, the assay of associated targets in partitions may lower the limit of detection (LOD) or otherwise increase assay sensitivity, accuracy, and/or specificity. The targets may represent spaced or overlapping regions of the same template and/or each may represent a region from a different complementary strand of the same template. In some embodiments, the associated targets may be provided by a type of biological particle, and the target content of partitions may be used to identify and quantify the type of biological particle in a sample.
摘要翻译： 用于执行具有相关目标的数字测定的系统，包括方法，装置和组合物。 在一些实施方案中，分区中相关靶标的测定可降低检测限（LOD）或以其它方式增加测定灵敏度，准确性和/或特异性。 靶可以表示相同模板的间隔或重叠区域和/或每个可以表示来自相同模板的不同互补链的区域。 在一些实施方案中，相关联的靶可以由一种生物颗粒提供，并且分隔物的目标含量可用于鉴定和定量样品中生物颗粒的类型。
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