Invention Application
- Patent Title: METHOD FOR PRODUCING PANCREATIC BLAST CELLS AND PANCREATIC DISEASE TREATMENT AGENT CONTAINING PANCREATIC BLAST CELLS
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Application No.: US15311759Application Date: 2015-05-20
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Publication No.: US20170175082A1Publication Date: 2017-06-22
- Inventor: Kenji OSAFUNE , Taro TOYODA
- Applicant: Kyoto University
- Priority: JP2014-105049 20140521
- International Application: PCT/JP2015/064529 WO 20150520
- Main IPC: C12N5/071
- IPC: C12N5/071 ; A61K35/39
Abstract:
Provided is a method for generating pancreatic bud cells, having the step of culturing PDX1+/NKX6.1− cells in a medium containing KGF, EGF and a BMP inhibitor. The culturing step may be performed in suspension cultures or in adherent cultures. When the cells are cultured in adherent cultures, the cells may be cultured in a medium further containing a ROCK inhibitor or a nonmuscle myosin II inhibitor.
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