In one aspect, a singulation chamber apparatus defines a volume having a receiving end and an opposing end. The receiving end may be couplable to an insect chamber for a plurality of ambulatory insects and define an opening to receive the plurality of ambulatory insects from the insect chamber. A surface of the chamber is ramped to provide a pathway between the receiving end and the opposing end such that the opposing end is elevated with respect to the first end via the ramped surface. The pathway has a width sized to accommodate a single-file line of the plurality of ambulatory insects traversing the ramped surface.