A honeycomb structure including prismatic columnar honeycomb segments and a bonding layer, wherein the bonding layer has rows in each of which bonding layer portions each disposed between two honeycomb segments arranged to face each other are arranged to extend from one point of a peripheral edge of a bonded body of the honeycomb segments to the other point thereof, in a cross section, the bonding layer of each of the-rows is disposed so that among the bonding layer portions arranged in one direction, an outermost circumference bonding layer portion and at least one of the other bonding layer portions are not superimposed on each other on an extension line of the one direction, and a ratio of shift of side surfaces of adjacent honeycomb segments to a length of one side of a side surface of honeycomb segments having the same cross-sectional shape is 10% or less.