发明申请
- 专利标题： PURIFICATION OF A SOLUBLE AND ACTIVE FORM OF ASPARTATE N-ACETYLTRANSFERASE
-
申请号： US15757837申请日： 2016-09-08
-
公开(公告)号： US20180340156A1公开(公告)日： 2018-11-29
- 发明人: Ronald E. Viola , Qinzhe Wang , Mojun Zhao , Gwenn Parungao
- 申请人： The University of Toledo
- 申请人地址： US OH Toledo
- 专利权人： The University of Toledo
- 当前专利权人： The University of Toledo
- 当前专利权人地址： US OH Toledo
- 国际申请： PCT/US2016/050737 WO 20160908
- 主分类号： C12N9/10
- IPC分类号： C12N9/10 ; C12N9/02 ; C07K14/245 ; C12Q1/48
摘要：
Fusion proteins that include N-acetylaspartate synthetase (ANAT) and at least one solubilizing partner, such as glutathione 5-transferase (GST), thioredoxin (TRX), or maltose binding protein (MBP), are described. Also described are methods of making the fusion proteins, methods of solubilizing the fusion proteins, methods of purifying the fusion proteins, and methods of using the fusion proteins.
信息查询
