发明申请
- 专利标题: NOVEL AND EFFICIENT METHOD FOR REPROGRAMMING BLOOD TO INDUCED PLURIPOTENT STEM CELLS
-
申请号: US16310360申请日: 2017-06-16
-
公开(公告)号: US20190194624A1公开(公告)日: 2019-06-27
- 发明人: Dhruv SAREEN , Loren A. ORNELAS , Clive SVENDSEN
- 申请人: CEDARS-SINAI MEDICAL CENTER
- 申请人地址: US CA Los Angeles
- 专利权人: Cedars-Sinai Medical Center
- 当前专利权人: Cedars-Sinai Medical Center
- 当前专利权人地址: US CA Los Angeles
- 国际申请: PCT/US2017/038041 WO 20170616
- 主分类号: C12N5/074
- IPC分类号: C12N5/074 ; C12N5/00
摘要:
Described herein are methods and compositions related to generation of induced pluripotent stem cells (iPSCs). Improved techniques for establishing highly efficient, reproducible reprogramming using non-integrating episomal plasmid vectors. Using the described reprogramming protocol, one is able to consistently reprogram non-T cells with close to 100% success from non-T cell or non-B cell sources. Further advantages include use of a defined reprogramming media E7 and using defined clinically compatible substrate recombinant human L-521. Generation of iPSCs from these blood cell sources allows for recapitulation of the entire genomic repertoire, preservation of genomic fidelity and enhanced genomic stability.