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公开(公告)号:US20190194624A1
公开(公告)日:2019-06-27
申请号:US16310360
申请日:2017-06-16
发明人: Dhruv SAREEN , Loren A. ORNELAS , Clive SVENDSEN
CPC分类号: C12N5/0696 , C12N5/0018 , C12N2500/12 , C12N2500/38 , C12N2501/115 , C12N2501/2302 , C12N2501/2303 , C12N2501/2306 , C12N2501/33 , C12N2501/998 , C12N2506/11 , C12N2533/52
摘要: Described herein are methods and compositions related to generation of induced pluripotent stem cells (iPSCs). Improved techniques for establishing highly efficient, reproducible reprogramming using non-integrating episomal plasmid vectors. Using the described reprogramming protocol, one is able to consistently reprogram non-T cells with close to 100% success from non-T cell or non-B cell sources. Further advantages include use of a defined reprogramming media E7 and using defined clinically compatible substrate recombinant human L-521. Generation of iPSCs from these blood cell sources allows for recapitulation of the entire genomic repertoire, preservation of genomic fidelity and enhanced genomic stability.