A yarn including a plurality of twists formed by twisting single fiber strand or multiple fiber strands; and fiber grooves, which are spaces formed between the twists, to provide three-dimensional growth spaces and migration paths for cells. Accordingly, a cell proliferation rate and cell viability may be enhanced by creating microenvironments suitable for migration, proliferation and differentiation of cultured cells. In addition, cell clusters having more uniform shapes may be easily implemented by forming the proliferation spaces and migration paths for the cultured cells as similar as possible to each other in each scaffold. Further, the cells cultured thereby can be cultured in a suitable shape and structure to be applied to an in vitro experimental model or transplanted into the body of an animal, and can be widely applied in various products used in a cell culture or tissue engineering field.