A method for maintaining a male sterile plant in a homozygous recessive state includes providing a first plant that includes homozygous recessive male sterility alleles, providing a second plant that includes homozygous recessive male sterility alleles the same as that in the first plant and a nucleotide construct in which the construct exists in a heterozygous state. The first nucleotide sequence of the nucleotide construct encodes a first protein that restores male fertility of the first plant after expression in the first plant. The second nucleotide sequence of the nucleotide construct allows for distinguishing the grains with or without the construct by observation through naked eyes or devices. The first nucleotide sequence and the second nucleotide sequence are tightly connected with each other and coexist in a plant. The method further includes fertilizing female gametes of the first plant with male gametes of the second plant.