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公开(公告)号：US10246723B2
公开(公告)日：2019-04-02
申请号：US14437633
申请日：2013-10-18
发明人： Jinsheng Lai , Haiming Zhao , Weibin Song , Yang Cui
摘要： A method for propagating a sterile male plant line includes: (a) providing a first plant that comprises a homozygous recessive male sterility allele; (b) providing a second plant comprising the homozygous recessive allele and a construct, where the construct exists in a heterozygous state and comprises i) a first nucleotide sequence that will restore the male fertility of the first plant after expression, and ii) a second nucleotide sequence that is able to affect the grain shape or the endosperm nutrient material composition in a heterozygous state, which allows for distinguishing the grains with or without said construct by observation through naked eyes or devices; the first nucleotide sequence and the second nucleotide sequence are tightly connected with each other and coexist in plants; and (c) fertilizing the female gametes of the first plant with the male gametes of the second plant.
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公开(公告)号：US11572573B2
公开(公告)日：2023-02-07
申请号：US16370628
申请日：2019-03-29
发明人： Jinsheng Lai , Haiming Zhao , Weibin Song , Yang Cui
摘要： A method for maintaining a male sterile plant in a homozygous recessive state includes providing a first plant that includes homozygous recessive male sterility alleles, providing a second plant that includes homozygous recessive male sterility alleles the same as that in the first plant and a nucleotide construct in which the construct exists in a heterozygous state. The first nucleotide sequence of the nucleotide construct encodes a first protein that restores male fertility of the first plant after expression in the first plant. The second nucleotide sequence of the nucleotide construct allows for distinguishing the grains with or without the construct by observation through naked eyes or devices. The first nucleotide sequence and the second nucleotide sequence are tightly connected with each other and coexist in a plant. The method further includes fertilizing female gametes of the first plant with male gametes of the second plant.
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公开(公告)号：US20190218572A1
公开(公告)日：2019-07-18
申请号：US16370628
申请日：2019-03-29
发明人： Jinsheng Lai , Haiming Zhao , Weibin Song , Yang Cui
CPC分类号： C12N15/8289 , A01H1/02 , A01H1/04 , C12N15/00 , C12N15/821 , C12N15/8218 , C12N15/8287
摘要： A method for maintaining a male sterile plant in a homozygous recessive state includes providing a first plant that includes homozygous recessive male sterility alleles, providing a second plant that includes homozygous recessive male sterility alleles the same as that in the first plant and a nucleotide construct in which the construct exists in a heterozygous state. The first nucleotide sequence of the nucleotide construct encodes a first protein that restores male fertility of the first plant after expression in the first plant. The second nucleotide sequence of the nucleotide construct allows for distinguishing the grains with or without the construct by observation through naked eyes or devices. The first nucleotide sequence and the second nucleotide sequence are tightly connected with each other and coexist in a plant. The method further includes fertilizing female gametes of the first plant with male gametes of the second plant.
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公开(公告)号：US20160145640A1
公开(公告)日：2016-05-26
申请号：US14437633
申请日：2013-10-18
发明人： Jinsheng Lai , Haiming Zhao , Weibin Song , Yang Cui
CPC分类号： C12N15/8289 , A01H1/02 , A01H1/04 , C12N15/00 , C12N15/821 , C12N15/8218 , C12N15/8287
摘要： A method for propagating a sterile male plant line includes: (a) providing a first plant that comprises a homozygous recessive male sterility allele; (b) providing a second plant comprising the homozygous recessive allele and a construct, where the construct exists in a heterozygous state and comprises i) a first nucleotide sequence that will restore the male fertility of the first plant after expression, and ii) a second nucleotide sequence that is able to affect the grain shape or the endosperm nutrient material composition in a heterozygous state, which allows for distinguishing the grains with or without said construct by observation through naked eyes or devices; the first nucleotide sequence and the second nucleotide sequence are tightly connected with each other and coexist in plants; and (c) fertilizing the female gametes of the first plant with the male gametes of the second plant.
摘要翻译： 用于繁殖无菌雄性植物系的方法包括：（a）提供包含纯合隐性雄性不育等位基因的第一植物; （b）提供包含纯合隐性等位基因和构建体的第二植物，其中构建体以杂合状态存在并且包含i）第一核苷酸序列，其将在表达后恢复第一植物的雄性能力，以及ii）第二种 能够影响杂种状态的颗粒形状或胚乳营养物质组成的核苷酸序列，其允许通过肉眼或器件观察来区分具有或不具有所述构建体的颗粒; 第一个核苷酸序列和第二个核苷酸序列彼此紧密连接并共存于植物中; 和（c）用第二植物的雄配子施肥第一植物的雌配子。
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