A wavelength conversion film including a phosphor layer and a light scattering layer is provided. The phosphor layer includes a first phosphor and a first substrate. The light scattering layer includes a plurality of titanium dioxide particles and a second substrate. The wavelength conversion film further includes a photoluminescence material and a plurality of nanoparticles. The photoluminescence material and the plurality of nanoparticles are located in at least one of the phosphor layer and the light scattering layer or respectively located in the phosphor layer and the light scattering layer. The wavelength conversion film of the invention may prevent the occurrence of photocatalytic effect and increase the light conversion efficiency.