发明申请
- 专利标题: DIGITAL COUNTING OF INDIVIDUAL MOLECULES BY STOCHASTIC ATTACHMENT OF DIVERSE LABELS
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申请号: US16551638申请日: 2019-08-26
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公开(公告)号: US20200224266A1公开(公告)日: 2020-07-16
- 发明人: Stephen P.A. Fodor , Glenn K. Fu
- 申请人: Becton, Dickinson and Company
- 主分类号: C12Q1/6874
- IPC分类号: C12Q1/6874 ; G16B25/00 ; G16B30/00 ; C12Q1/6809 ; C12Q1/686 ; C12N15/10 ; C12Q1/6869 ; C12Q1/6837 ; C12Q1/6876 ; C12Q1/6851
摘要:
Compositions, methods and kits are disclosed for high-sensitivity single molecule digital counting by the stochastic labeling of a collection of identical molecules by attachment of a diverse set of labels. Each copy of a molecule randomly chooses from a non-depleting reservoir of diverse labels. Detection may be by a variety of methods including hybridization based or sequencing. Molecules that would otherwise be identical in information content can be labeled to create a separately detectable product that is unique or approximately unique in a collection. This stochastic transformation relaxes the problem of counting molecules from one of locating and identifying identical molecules to a series of binary digital questions detecting whether preprogrammed labels are present. The methods may be used, for example, to estimate the number of separate molecules of a given type or types within a sample.
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