Provided are a chitin whisker-enhanced hyaluronic acid cell scaffold and a preparation method thereof. Components of the cell scaffold include chitin whiskers and cross-linked hyaluronic acid. The chitin whisker-enhanced hyaluronic acid cell scaffold is obtained by dispersing chitin whiskers into deionized water using ultrasound, followed by addition of hyaluronic acid and uniform mixing to obtain an aqueous solution, adjusting a pH value of the aqueous solution to be in a range of 4.0 to 6.0, subjecting the aqueous solution to a cross-linking reaction, dialyzing a reaction product in a phosphate buffer solution, and freeze drying a resulting product. The chitin whisker-enhanced hyaluronic acid cell scaffold and the preparation method thereof can increase a mechanical property and a resistance to degradation of a scaffold material and expand an application scope of hyaluronic acid cell scaffolds.