- 专利标题： METHOD FOR PRODUCING COMPOUND WITH MODIFIED MOTHER NUCLEUS
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申请号： US17426621申请日： 2020-01-30
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公开(公告)号： US20220090100A1公开(公告)日： 2022-03-24
- 发明人: Kazuo SHINYA , Haruo IKEDA , Mamoru KOMATSU , Junko HASHIMOTO , Ikuko KOZONE , Takuya HASHIMOTO , Kei KUDO
- 申请人： JAPAN BIOLOGICAL INFORMATICS CONSORTIUM
- 申请人地址： JP Tokyo
- 专利权人： JAPAN BIOLOGICAL INFORMATICS CONSORTIUM
- 当前专利权人： JAPAN BIOLOGICAL INFORMATICS CONSORTIUM
- 当前专利权人地址： JP Tokyo
- 优先权： JP2019-016531 20190131
- 国际申请： PCT/JP2020/003309 WO 20200130
- 主分类号： C12N15/76
- IPC分类号： C12N15/76 ; C07K7/54 ; C12P17/18 ; C12N15/11 ; C12N9/22
摘要：
The present invention provides a method for producing a modified compound, including the following steps: (1) a step of cleaving in vitro using a CRISPR/Cas9 system, a target site in a gene cluster involved in the biosynthesis of a compound, (2) a step of connecting using Gibson assembly in vitro the gene cluster cleaved in step (1) and a polynucleotide for modification, and (3) a step of expressing the modified gene cluster obtained in step (2) in a microorganism expression system.
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