专利检索 ap:("JAPAN BIOLOGICAL INFORMATICS CONSORTIUM") AND inv:"Haruo IKEDA" 第 1 页

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发明申请
METHOD FOR PRODUCING COMPOUND WITH MODIFIED MOTHER NUCLEUS 有权

公开(公告)号：US20220090100A1

公开(公告)日：2022-03-24

申请号：US17426621

申请日：2020-01-30

申请人： JAPAN BIOLOGICAL INFORMATICS CONSORTIUM

发明人： Kazuo SHINYA , Haruo IKEDA , Mamoru KOMATSU , Junko HASHIMOTO , Ikuko KOZONE , Takuya HASHIMOTO , Kei KUDO

IPC分类号： C12N15/76 , C07K7/54 , C12P17/18 , C12N15/11 , C12N9/22

摘要： The present invention provides a method for producing a modified compound, including the following steps: (1) a step of cleaving in vitro using a CRISPR/Cas9 system, a target site in a gene cluster involved in the biosynthesis of a compound, (2) a step of connecting using Gibson assembly in vitro the gene cluster cleaved in step (1) and a polynucleotide for modification, and (3) a step of expressing the modified gene cluster obtained in step (2) in a microorganism expression system.