- 专利标题: METHOD OF COLLECTING NUCLEIC ACID AND KIT FOR COLLECTION OF NUCLEIC ACID
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申请号: US17287194申请日: 2019-10-21
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公开(公告)号: US20220090165A1公开(公告)日: 2022-03-24
- 发明人: Shota Sekiguchi , Takahiro Motoshiromizu , Masateru Ito
- 申请人: Toray Industries, Inc.
- 申请人地址: JP Tokyo
- 专利权人: Toray Industries, Inc.
- 当前专利权人: Toray Industries, Inc.
- 当前专利权人地址: JP Tokyo
- 优先权: JP2018-199267 20181023
- 国际申请: PCT/JP2019/041410 WO 20191021
- 主分类号: C12Q1/6806
- IPC分类号: C12Q1/6806 ; G01N30/14 ; G01N30/88
摘要:
A method of collecting a nucleic acid from a sample containing a nucleic acid using a support of aluminum oxide having a surface where a water-soluble neutral polymer is adsorbed, the method including steps a to c: step a: a step of bringing the support into contact with the sample containing a nucleic acid to adsorb the nucleic acid on the support; step b: a step of bringing the support on which the nucleic acid is adsorbed into contact with a solution A containing 1 mM or more and 40 mM or less of a chelating agent; and step c: after the step b, a step of bringing the support on which the nucleic acid is adsorbed into contact with a solution B containing 50 mM or more of a chelating agent to elute the nucleic acid.
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