Invention Application
- Patent Title: METHOD FOR MEASUREMENT OF LIVE-CELL PARAMETERS FOLLOWED BY MEASUREMENT OF GENE AND PROTEIN EXPRESSION
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Application No.: US17538353Application Date: 2021-11-30
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Publication No.: US20220091100A1Publication Date: 2022-03-24
- Inventor: Clifford Anderson , Dmitry Derkach , Deirdre Meldrum , Laimonas Kelbauskas
- Applicant: Arizona Board of Regents on behalf of Arizona State University
- Applicant Address: US AZ Scottsdale
- Assignee: Arizona Board of Regents on behalf of Arizona State University
- Current Assignee: Arizona Board of Regents on behalf of Arizona State University
- Current Assignee Address: US AZ Scottsdale
- Main IPC: G01N33/50
- IPC: G01N33/50 ; C12Q1/686 ; G01N21/64

Abstract:
A method for analyzing cells through measurement of live-cell parameters followed by measurement of gene and protein expression is disclosed herein. The method comprises measuring one or more live-cell parameters for a plurality of cells contained in at least one liquid in a plurality of isolated microchambers of a microarray device. The method further comprises removing a lid bounding the plurality of isolated microchambers. The method further comprises microdispensing a quantity of lysate into each microchamber of the plurality of isolated microchambers. The method further comprises microdispensing a quantity of reverse transcription polymerase chain reaction mix into each microchamber of the plurality of isolated microchambers. The method further comprises microdispensing a quantity of oil into each microchamber of the plurality of isolated microchambers. The method further comprises incorporating the microarray device into a thermal cycling apparatus with a window permitting epifluorescence imaging of the plurality of isolated microchambers.
Information query
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