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公开(公告)号：US11136614B2
公开(公告)日：2021-10-05
申请号：US15757712
申请日：2016-10-07
Inventor： Jacob Messner , Clifford Anderson , Honor Glenn , Kristen Lee , Mark Richards , Laimonas Kelbauskas , Kimberly Bussey , Deirdre Meldrum
IPC: C12Q1/24 , C12M1/00 , C12M1/32 , G01N33/569
Abstract: Methods for seeding live cells onto spatially defined regions of a substrate including multiple features (e.g., microwells or other microenvironments) utilize a stencil embodied in a hole-defining sacrificial film. A sacrificial film devoid of holes may be applied over features of a substrate, and a hole generating mechanism (e.g., hot needle or laser) aligned with features may be used to define holes in the film. Alternatively, holes may be predefined in a sacrificial film to form a stencil, and the stencil may be assembled to the substrate with the holes registered with features thereof. Thereafter, cells are seeded through holes in the film. Seeded cells are subject to incubation, further processing, and/or performance of one or more assays, and the hole-defining sacrificial film (stencil) may be removed.
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公开(公告)号：US20220091100A1
公开(公告)日：2022-03-24
申请号：US17538353
申请日：2021-11-30
Inventor： Clifford Anderson , Dmitry Derkach , Deirdre Meldrum , Laimonas Kelbauskas
Abstract: A method for analyzing cells through measurement of live-cell parameters followed by measurement of gene and protein expression is disclosed herein. The method comprises measuring one or more live-cell parameters for a plurality of cells contained in at least one liquid in a plurality of isolated microchambers of a microarray device. The method further comprises removing a lid bounding the plurality of isolated microchambers. The method further comprises microdispensing a quantity of lysate into each microchamber of the plurality of isolated microchambers. The method further comprises microdispensing a quantity of reverse transcription polymerase chain reaction mix into each microchamber of the plurality of isolated microchambers. The method further comprises microdispensing a quantity of oil into each microchamber of the plurality of isolated microchambers. The method further comprises incorporating the microarray device into a thermal cycling apparatus with a window permitting epifluorescence imaging of the plurality of isolated microchambers.
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3.发明申请公开(公告)号：US20200049694A1
公开(公告)日：2020-02-13
申请号：US15774563
申请日：2016-11-16
Inventor： Clifford Anderson , Dmitry Derkach , Deirdre Meldrum , Laimonas Kelbauskas
Abstract: A method for analyzing cells through measurement of live-cell parameters followed by measurement of gene and protein expression is disclosed herein. The method comprises measuring one or more live-cell parameters for a plurality of cells contained in at least one liquid in a plurality of isolated microchambers of a microarray device. The method further comprises removing a lid bounding the plurality of isolated microchambers. The method further comprises microdispensing a quantity of lysate into each microchamber of the plurality of isolated microchambers. The method further comprises microdispensing a quantity of reverse transcription polymerase chain reaction mix into each microchamber of the plurality of isolated microchambers. The method further comprises microdispensing a quantity of oil into each microchamber of the plurality of isolated microchambers. The method further comprises incorporating the microarray device into a thermal cycling apparatus with a window permitting epifluorescence imaging of the plurality of isolated microchambers.
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Patent Agency Ranking
公开(公告)号：US10940476B2
公开(公告)日：2021-03-09
申请号：US16095375
申请日：2017-04-21
Inventor： Laimonas Kelbauskas , Honor Glenn , Jeff Houkal , Clifford Anderson , Yanqing Tian , Fengyu Su , Deirdre Meldrum
Abstract: A device for high-throughput multi-parameter functional profiling of the same cells in multicellular settings and in isolation is provided. In certain aspects, an integrated microfluidic device for multi-parameter metabolic and other phenotypic profiling of live biological cells is useable with: 1) multicellular clusters or small biopsy tissue samples, 2) cultures of the constituent cells obtained after cluster/tissue dissociation, and 3) the same constituent single cells in isolation. The approach enables study of the effects of multicellular complexity, such as in response to treatment, pathogens, stress, or other factors concerning disease origination and progression. Measurements may be performed on single cells or multicellular populations or tissues in the same assay at the same time.
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公开(公告)号：US20180334700A1
公开(公告)日：2018-11-22
申请号：US15757712
申请日：2016-10-07
Inventor： Jacob Messner , Clifford Anderson , Honor Glenn , Kristen Lee , Mark Richards , Laimonas Kelbauskas , Kimberly Bussey , Deirdre Meldrum
IPC: C12Q1/24 , G01N33/569
CPC classification number: C12Q1/24 , C12M1/00 , C12M23/12 , G01N33/56966
Abstract: Methods for seeding live cells onto spatially defined regions of a substrate including multiple features (e.g., microwells or other microenvironments) utilize a stencil embodied in a hole-defining sacrificial film. A sacrificial film devoid of holes may be applied over features of a substrate, and a hole generating mechanism (e.g., hot needle or laser) aligned with features may be used to define holes in the film. Alternatively, holes may be predefined in a sacrificial film to form a stencil, and the stencil may be assembled to the substrate with the holes registered with features thereof. Thereafter, cells are seeded through holes in the film. Seeded cells are subject to incubation, further processing, and/or performance of one or more assays, and the hole-defining sacrificial film (stencil) may be removed.
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公开(公告)号：US11369966B2
公开(公告)日：2022-06-28
申请号：US15760984
申请日：2016-09-16
Inventor： Clifford Anderson , Kristen Lee , Jacob Messner , Laimonas Kelbauskas , Benjamin Ueberroth , Yanqing Tian , Deirdre Meldrum
IPC: B01L3/00
Abstract: A multi-layer sealing structure for sealing a microwell array defined in or on a substrate includes at least one front compliant layer, a back compliant layer, and a flexural layer arranged between the at least one front compliant layer and the back compliant layer, wherein the at least one front compliant layer is closer than the back compliant layer to microwells of the microwell array. One or more front compliant layers may be optically reflective and/or may embody a sensor layer. The back compliant layer may include an adhesive or various types of rubber, and the flexural layer may include a polymeric material or metal. A multi-layer sealing structure may be separated from a microwell array by peeling. A multi-layer sealing structure allows local disruption of sealing where particle contaminants are present without compromising the sealing performance of an entire microwell array, and without requiring a large sealing force.
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公开(公告)号：US20180264468A1
公开(公告)日：2018-09-20
申请号：US15760984
申请日：2016-09-16
Inventor： Clifford Anderson , Kristen Lee , Jacob Messner , Laimonas Kelbauskas , Benjamin Ueberroth , Yanqing Tian , Deirdre Meldrum
IPC: B01L3/00
CPC classification number: B01L3/50853 , B01L2200/025 , B01L2200/0689 , B01L2200/141 , B01L2200/142 , B01L2300/0627 , B01L2300/0829 , B01L2300/123 , B01L2300/16
Abstract: A multi-layer sealing structure for sealing a microwell array defined in or on a substrate includes at least one front compliant layer, a back compliant layer, and a flexural layer arranged between the at least one front compliant layer and the back compliant layer, wherein the at least one front compliant layer is closer than the back compliant layer to microwells of the microwell array. One or more front compliant layers may be optically reflective and/or may embody a sensor layer. The back compliant layer may include an adhesive or various types of rubber, and the flexural layer may include a polymeric material or metal. A multi-layer sealing structure may be separated from a microwell array by peeling. A multi-layer sealing structure allows local disruption of sealing where particle contaminants are present without compromising the sealing performance of an entire microwell array, and without requiring a large sealing force.
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8.发明申请公开(公告)号：US20190126275A1
公开(公告)日：2019-05-02
申请号：US16095375
申请日：2017-04-21
Inventor： Laimonas Kelbauskas , Honor Glenn , Jeff Houkal , Clifford Anderson , Yanqing Tian , Fengyu Su , Deirdre Meldrum
Abstract: A device for high-throughput multi-parameter functional profiling of the same cells in multicellular settings and in isolation is provided. In certain aspects, an integrated microfluidic device for multi-parameter metabolic and other phenotypic profiling of live biological cells is useable with: 1) multicellular clusters or small biopsy tissue samples, 2) cultures of the constituent cells obtained after cluster/tissue dissociation, and 3) the same constituent single cells in isolation. The approach enables study of the effects of multicellular complexity, such as in response to treatment, pathogens, stress, or other factors concerning disease origination and progression. Measurements may be performed on single cells or multicellular populations or tissues in the same assay at the same time.
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