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公开(公告)号：US20220091100A1
公开(公告)日：2022-03-24
申请号：US17538353
申请日：2021-11-30
Inventor： Clifford Anderson , Dmitry Derkach , Deirdre Meldrum , Laimonas Kelbauskas
Abstract: A method for analyzing cells through measurement of live-cell parameters followed by measurement of gene and protein expression is disclosed herein. The method comprises measuring one or more live-cell parameters for a plurality of cells contained in at least one liquid in a plurality of isolated microchambers of a microarray device. The method further comprises removing a lid bounding the plurality of isolated microchambers. The method further comprises microdispensing a quantity of lysate into each microchamber of the plurality of isolated microchambers. The method further comprises microdispensing a quantity of reverse transcription polymerase chain reaction mix into each microchamber of the plurality of isolated microchambers. The method further comprises microdispensing a quantity of oil into each microchamber of the plurality of isolated microchambers. The method further comprises incorporating the microarray device into a thermal cycling apparatus with a window permitting epifluorescence imaging of the plurality of isolated microchambers.
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2.发明申请公开(公告)号：US20200049694A1
公开(公告)日：2020-02-13
申请号：US15774563
申请日：2016-11-16
Inventor： Clifford Anderson , Dmitry Derkach , Deirdre Meldrum , Laimonas Kelbauskas
Abstract: A method for analyzing cells through measurement of live-cell parameters followed by measurement of gene and protein expression is disclosed herein. The method comprises measuring one or more live-cell parameters for a plurality of cells contained in at least one liquid in a plurality of isolated microchambers of a microarray device. The method further comprises removing a lid bounding the plurality of isolated microchambers. The method further comprises microdispensing a quantity of lysate into each microchamber of the plurality of isolated microchambers. The method further comprises microdispensing a quantity of reverse transcription polymerase chain reaction mix into each microchamber of the plurality of isolated microchambers. The method further comprises microdispensing a quantity of oil into each microchamber of the plurality of isolated microchambers. The method further comprises incorporating the microarray device into a thermal cycling apparatus with a window permitting epifluorescence imaging of the plurality of isolated microchambers.
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公开(公告)号：US20180318835A1
公开(公告)日：2018-11-08
申请号：US16035464
申请日：2018-07-13
Inventor： Rhett Martineau , Jeff Houkal , Shih-Hui Chao , Weimin Gao , Shufang Ci , Deirdre Meldrum
CPC classification number: B01L3/502715 , B01L3/502723 , B01L3/50273 , B01L7/52 , B01L2200/0605 , B01L2200/0642 , B01L2200/0647 , B01L2200/0684 , B01L2300/044 , B01L2300/0636 , B01L2300/0681 , B01L2300/0803 , B01L2300/0829 , B01L2300/0851 , B01L2300/0864 , B01L2300/0883 , B01L2300/161 , B01L2400/049 , B01L2400/0683
Abstract: The invention relates to microfluidic devices and array disks for “one-pot” isolated chemical reactions. The array disks comprise a plurality of sectors in which each sector comprises one microfluidic device. The microfluidic devices comprise a fluid delivery channel and an array of wells wherein the fluid delivery channel delivery fluid into the wells in a serpentine arrangement. In some embodiments, the fluid delivery channel is directly above the array of wells. In other embodiments, the fluid delivery channel is offset from the array of wells so that side channels branching from the fluid delivery channel delivers fluid into the wells. The well of the microfluidic device comprises a gas-permeable membrane that forms the floor, well, or at least a portion of the floor or wall of the well. In preferred embodiments, the well is cylindrical.
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公开(公告)号：US11364502B2
公开(公告)日：2022-06-21
申请号：US15774558
申请日：2016-11-14
Inventor： Deirdre Meldrum , Laimonas Kelbauskas , Wacey Teller , Meryl Rodrigues , Hong Wang , Ganquan Song , Yanqing Tian , Fengyu Su , Xiangxing Kong , Liqiang Zhang
Abstract: A microfluidic device includes a first substrate including at least one microfluidic channel and a plurality of microwells, as well as a cooperating second substrate defining multiple split-walled cell trap structures that are registered with and disposed within the plurality of microwells. A method for performing an assay includes flowing cells and a first aqueous medium into a plurality of microwells of a microfluidic device, wherein each microwell includes a cell trap structure configured to trap at least one cell. The method further comprises flowing a nonpolar fluid with low permeability for analytes of interest through a microfluidic channel to flush a portion of the first aqueous medium from the microfluidic channel while retaining another portion of the first aqueous medium and at least one cell within each microwell. Surface tension at a non-polar/polar medium interface prevents molecule exchange between interior and exterior portions of microwells.
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公开(公告)号：US10940476B2
公开(公告)日：2021-03-09
申请号：US16095375
申请日：2017-04-21
Inventor： Laimonas Kelbauskas , Honor Glenn , Jeff Houkal , Clifford Anderson , Yanqing Tian , Fengyu Su , Deirdre Meldrum
Abstract: A device for high-throughput multi-parameter functional profiling of the same cells in multicellular settings and in isolation is provided. In certain aspects, an integrated microfluidic device for multi-parameter metabolic and other phenotypic profiling of live biological cells is useable with: 1) multicellular clusters or small biopsy tissue samples, 2) cultures of the constituent cells obtained after cluster/tissue dissociation, and 3) the same constituent single cells in isolation. The approach enables study of the effects of multicellular complexity, such as in response to treatment, pathogens, stress, or other factors concerning disease origination and progression. Measurements may be performed on single cells or multicellular populations or tissues in the same assay at the same time.
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Patent Agency Ranking
公开(公告)号：US20200063197A1
公开(公告)日：2020-02-27
申请号：US16611799
申请日：2018-05-15
Inventor： Deirdre Meldrum , Shufang Ci , Shih-Hui (Joseph) Chao , Rhett Martineau , Weimin Gao
Abstract: The present disclosure relates to real-time quantification using loop-mediated isothermal amplification, in particular real-time colorimetric reverse transcription quantitative loop-mediated isothermal amplification (RT-qLAMP). In some embodiments, RT-qLAMP is used to diagnose the presence of and also quantitate the amount of Zika virus in a sample.
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7.发明申请公开(公告)号：US20200047182A1
公开(公告)日：2020-02-13
申请号：US15774558
申请日：2016-11-14
Inventor： Deirdre Meldrum , Laimonas Kelbauskas , Wacey Teller , Meryl Rodrigues , Hong Wang , Ganquan Song , Yanqing Tian , Fengyu Su , Xiangxing Kong , Liqiang Zhang
IPC: B01L3/00
Abstract: A microfluidic device includes a first substrate including at least one microfluidic channel and a plurality of microwells, as well as a cooperating second substrate defining multiple split-walled cell trap structures that are registered with and disposed within the plurality of microwells. A method for performing an assay includes flowing cells and a first aqueous medium into a plurality of microwells of a microfluidic device, wherein each microwell includes a cell trap structure configured to trap at least one cell. The method further comprises flowing a nonpolar fluid with low permeability for analytes of interest through a microfluidic channel to flush a portion of the first aqueous medium from the microfluidic channel while retaining another portion of the first aqueous medium and at least one cell within each microwell. Surface tension at a non-polar/polar medium interface prevents molecule exchange between interior and exterior portions of microwells.
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公开(公告)号：US20190153005A1
公开(公告)日：2019-05-23
申请号：US16314753
申请日：2017-07-14
Inventor： Xiangxing Kong , Yanqing Tian , Fengyu Su , Liqiang Zhang , Deirdre Meldrum
IPC: C07F9/6596 , G01N33/50 , G01N33/58 , G01N33/68
CPC classification number: C07F9/6596 , A61K31/69 , C07D498/02 , C07D498/08 , C07D498/18 , G01N33/5079 , G01N33/582 , G01N33/6872
Abstract: Mitochondria-targeting potassium sensors and method(s) for making such sensors. The sensor shows a response to potassium and displays a 130-fold dynamic range of fluorescence intensity and high brightness. The sensors response to potassium concentrations was demonstrated to be unaffected by cellular pH value and/or concentrations of other ions. The sensors can be used for monitoring the mitochondrial potassium efflux/influx.
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公开(公告)号：US20180334700A1
公开(公告)日：2018-11-22
申请号：US15757712
申请日：2016-10-07
Inventor： Jacob Messner , Clifford Anderson , Honor Glenn , Kristen Lee , Mark Richards , Laimonas Kelbauskas , Kimberly Bussey , Deirdre Meldrum
IPC: C12Q1/24 , G01N33/569
CPC classification number: C12Q1/24 , C12M1/00 , C12M23/12 , G01N33/56966
Abstract: Methods for seeding live cells onto spatially defined regions of a substrate including multiple features (e.g., microwells or other microenvironments) utilize a stencil embodied in a hole-defining sacrificial film. A sacrificial film devoid of holes may be applied over features of a substrate, and a hole generating mechanism (e.g., hot needle or laser) aligned with features may be used to define holes in the film. Alternatively, holes may be predefined in a sacrificial film to form a stencil, and the stencil may be assembled to the substrate with the holes registered with features thereof. Thereafter, cells are seeded through holes in the film. Seeded cells are subject to incubation, further processing, and/or performance of one or more assays, and the hole-defining sacrificial film (stencil) may be removed.
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公开(公告)号：US20160237476A1
公开(公告)日：2016-08-18
申请号：US15135150
申请日：2016-04-21
Inventor： Deirdre Meldrum , Shih-Hui (Joseph) Chao , Thai Tran , Laimonas Kelbauskas , Jeff Houkal , Andrew Hatch , Weimin Gao , David Richardson
IPC: C12Q1/68
CPC classification number: C12Q1/6806 , B01L3/0293 , B01L3/502761 , B01L2200/0668 , B01L2300/0816 , B01L2300/0829 , B01L2300/0864 , C12M47/06 , C12Q1/6841 , C12Q1/6848 , C12Q1/686 , G01N1/286 , G01N2001/2886 , C12Q2547/101
Abstract: Systems and methods for in situ laser lysis for analysis of biological tissue (live, fixed, frozen or otherwise preserved) at single cell resolution in 3D. For example, a system and method for lysing individual cells in situ, including the steps of capturing a tissue sample comprising a cellular content, subjecting the tissue sample to a stream of continuous fluid flow, lysing a selected area of the tissue sample with a laser, thereby releasing at least a portion of the cellular content from the tissue sample, recovering at least one target molecule from the cellular content in the stream, and processing at least one target molecule is provided. The system collects cellular contents, performs highly multiplexed (RT-qPCR or RNA-seq), and sequentially (cell-by-cell) reconstructs a 3D spatial map of mRNA expression of the tissue with a large number of genes. A 3D spatial map of the DNA, RNA, and/or proteins can be generated for each cell in the tissue.
Abstract translation: 用于原位激光裂解的系统和方法，用于分析3D中单细胞分辨率的生物组织（活，固定，冷冻或以其他方式保存）。 例如，用于原位裂解单个细胞的系统和方法，包括以下步骤：捕获包含细胞内容物的组织样品，使组织样品经受连续流体流的流动，用激光裂解组织样品的选定区域 从而从组织样品释放至少一部分细胞含量，从流中的细胞含量回收至少一种靶分子，并且提供至少一种靶分子的加工。 系统收集细胞内容，进行高度多重化（RT-qPCR或RNA-seq），并依次（逐个细胞）重构具有大量基因的组织的mRNA表达的3D空间图。 可以为组织中的每个细胞产生DNA，RNA和/或蛋白质的3D空间图。
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