Provided is an apparatus for cell particle sorting based on microfluidic-chip flow, by using a design in which Dean flow focusing occurring in a spiral channel and hydrodynamic filtration are coupled. The apparatus comprises a first substrate including a spiral channel having an inner surface and an outer surface based on a radius of curvature, a sample solution inlet, a medium inlet, and a spiral inner-outlet and a spiral outer-outlet both for discharging the particles, and a second substrate including a main channel in which the sample solution discharged from the first substrate and passing through an inter-substrate way flows and a cut-off width WC is set, a side channel allowing a medium introduced into the medium inlet to flow to focus the sample solution on a sidewall of the main channel, a plurality of branch channels connected to the sidewall of main channel and configured to receive the particles from the main channel, a main channel outlet, and at least one branch channel outlet.