A display substrate includes: a base substrate; a light-emitting unit in each pixel region and including a first electrode, an organic light-emitting layer, and a second electrode sequentially disposed in a direction away from the base substrate; an auxiliary conductive layer between the light-emitting unit and the base substrate; a pixel circuit in each pixel region and including a driving transistor. The auxiliary conductive layer is on a side of the pixel circuit away from the base substrate, the second electrode has a portion extending out of the pixel region and coupling to the auxiliary conductive layer through a via hole not overlapping the pixel region, the auxiliary conductive layer is insulated and spaced apart from the first electrode and has a mesh or chain shape, and a material of the auxiliary conductive layer is the same as a material of the first and second electrodes of the driving transistor.