发明申请
- 专利标题： INDUSTRIAL FERMENTATION PROCESS FOR BACILLUS USING DEFINED MEDIUM AND MAGNESIUM FEED
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申请号： US17432204申请日： 2020-02-18
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公开(公告)号： US20220186177A1公开(公告)日： 2022-06-16
- 发明人: Andreas Daub , Aydin Golabgir Anbarani , Tobias Klein , Stephan Freyer , Thomas Kaeding , Max Fabian Felle , Paul Igor Costea
- 申请人： BASF SE
- 申请人地址： DE Ludwigshafen
- 专利权人： BASF SE
- 当前专利权人： BASF SE
- 当前专利权人地址： DE Ludwigshafen
- 优先权： EP19158372.3 20190220,EP19215651.1 20191212
- 国际申请： PCT/EP2020/054172 WO 20200218
- 主分类号： C12N1/38
- IPC分类号： C12N1/38 ; C12P21/02 ; C12N1/20
摘要：
The present invention is directed to an industrial fermentation process for cultivating a Bacillus cell in a chemically defined fermentation medium and a method for producing a protein of inter-est comprising the steps of providing a chemically defined fermentation medium, inoculating the fermentation medium with a Bacillus cell comprising a gene encoding a protein of interest, cultivating the Bacillus cell in the fermentation medium under conditions conductive for the growth of the Bacillus cell and the expression of the protein of interest, wherein the cultivation of the Bacillus cell comprises the addition of one or more feed solutions comprising one or more chemically defined carbon sources and magnesium ions to the fermentation medium.