公开(公告)号：US20220186234A1

公开(公告)日：2022-06-16

申请号：US17432203

申请日：2020-02-18

申请人： BASF SE

发明人： Andreas Daub ,  Aydin Golabgir Anbarani ,  Tobias Klein ,  Stephan Freyer ,  Thomas Kaeding ,  Max Fabian Felle ,  Paul Igor Costea

IPC分类号： C12N15/75 ,  C12N1/20 ,  C12P17/16 ,  C12N9/54

摘要： The present invention is directed to an industrial fermentation process for cultivating a Bacillus cell in a chemically defined fermentation medium and a method for producing a protein of interest comprising the steps of providing a chemically defined fermentation medium, inoculating the fermentation medium with a Bacillus cell comprising a gene encoding a protein of interest, cultivating the Bacillus cell in the fermentation medium under conditions conductive for the growth of the Bacillus cell and the expression of the protein of interest, wherein the cultivation of the Bacillus cell comprises the addition of one or more feed solutions comprising one or more chemically defined carbon sources and one or more trace element ions to the fermentation medium.

公开(公告)号：US20220186177A1

公开(公告)日：2022-06-16

申请号：US17432204

申请日：2020-02-18

申请人： BASF SE

发明人： Andreas Daub ,  Aydin Golabgir Anbarani ,  Tobias Klein ,  Stephan Freyer ,  Thomas Kaeding ,  Max Fabian Felle ,  Paul Igor Costea

IPC分类号： C12N1/38 ,  C12P21/02 ,  C12N1/20

摘要： The present invention is directed to an industrial fermentation process for cultivating a Bacillus cell in a chemically defined fermentation medium and a method for producing a protein of inter-est comprising the steps of providing a chemically defined fermentation medium, inoculating the fermentation medium with a Bacillus cell comprising a gene encoding a protein of interest, cultivating the Bacillus cell in the fermentation medium under conditions conductive for the growth of the Bacillus cell and the expression of the protein of interest, wherein the cultivation of the Bacillus cell comprises the addition of one or more feed solutions comprising one or more chemically defined carbon sources and magnesium ions to the fermentation medium.

公开(公告)号：US20190024038A1

公开(公告)日：2019-01-24

申请号：US16060626

申请日：2016-12-08

申请人： BASF SE

发明人： Katrin Treier-Marxen ,  Petra Deckert ,  Andreas Schaedler ,  Vaidotas Navickas ,  Volker Wengert ,  Thomas Kaeding

IPC分类号： C12N1/02 ,  B01D61/14 ,  B01D61/16 ,  C07K1/14 ,  C07K1/30 ,  C12P21/02

摘要： The present invention is directed to a method of purifying a protein of interest from the particulate matter of a fermentation broth comprising the step of purifying the protein of interest from the particulate matter of the fermentation broth, wherein the step of purifying the protein of interest from the particulate matter of the fermentation broth comprises one or more washing steps, which comprises contacting the particulate matter with a washing solution comprising one or more conditions that favor the solubilization of the protein of interest and/or the desorption of the protein of interest from the particulate matter.

公开(公告)号：US20190292514A1

公开(公告)日：2019-09-26

申请号：US16317338

申请日：2017-07-12

申请人： BASF SE

发明人： Kerstin Hage ,  Carsten Schwalb ,  Gunter Steigelmann ,  Thomas Kaeding ,  Sebastian Briechle

IPC分类号： C12N1/20 ,  C12N1/38

摘要： The present invention is directed to a fermentation medium and a fermentation method using said fermentation medium wherein the fermentation medium comprising a basal medium and a chelating agent having the formula (I) R1—CH(COOX1)—N(CH2COOX1)2, wherein R1 is selected from hydrogen, linear or branched C1-C4-alkyl, phenyl, benzyl, CH2OH, and CH2CH2COOX1, X1 is (MxH1-x), M being a monovalent cation, preferably being selected from ammonium or alkali metal or combinations of at least two of the foregoing, x is in the range of from zero to 1, wherein x is an average value.

公开(公告)号：US09598428B2

公开(公告)日：2017-03-21

申请号：US14760302

申请日：2014-01-15

申请人： BASF SE

发明人： Melanie Bonnekessel ,  Wolfgang Reichert ,  Ralf Hoock ,  Thomas Kaeding ,  Christopher Koradin ,  Andreas Pletsch ,  Manfred Ehresmann ,  Hartwig Schroeder

IPC分类号： A01N43/90 ,  A01N53/00 ,  C07D493/04

CPC分类号： C07D493/04 ,  A01N43/90 ,  A01N53/00

摘要： The present invention relates to a method for preparing the pyripyropene compound of the formula (I) which method comprises the following steps: i) subjecting a pyripyropene compound of formula Pyripyropene A to an alkaline hydrolysis to yield a 1,7,11 -trideacetylpyripyropene A, ii) reacting 1,7,11-trideacetylpyripyropene A obtained in step i) with cyclopropane carbonyl chloride to yield a raw product containing the pyripyropene compound of formula (I); iii) subjecting the raw product of step ii) to crystallization to yield a crystalline pyripyropene compound of formula (I) and a mother liquor; and iv) recycling the mother liquor or a pyripyropene compound containing fraction thereof to the alkaline hydrolysis of step i).

公开(公告)号：US20220340865A1

公开(公告)日：2022-10-27

申请号：US17620786

申请日：2020-06-29

申请人： BASF SE

发明人： Thomas Kaeding ,  Kerstin Hage ,  Rodolfo Ham-Zhu

IPC分类号： C12N1/20

摘要： The present invention is directed to a method of preparing a fermentation medium comprising the steps of a) mixing components of a fermentation medium comprising a complex nutrient source in water, wherein the complex nutrient source is in an amount of 0.5-30% w/v of the fermentation medium; b) adjusting the pH of the fermentation medium obtained in step a) to a pH between pH 3.0 and pH 6.7; c) subjecting the fermentation medium subsequently to a heat treatment and thereafter adjusting the pH to a pH value suitable for cell cultivation. The present invention is further directed to a method a fermentation process comprising cultivation of the cells in the fermentation medium of the invention and the production of a compound of interest. The present invention is further directed to a method for reducing color formation and viscosity during fermentation medium preparation and during the fermentation process.

公开(公告)号：US10150777B2

公开(公告)日：2018-12-11

申请号：US14778788

申请日：2014-03-03

申请人： BASF SE

发明人： Burkhard Ernst ,  Manfred Ehresmann ,  Christopher Koradin ,  Andreas Pletsch ,  Melanie Bonnekessel ,  Thomas Kaeding ,  Karin Schein-Albrecht ,  Stefanie Demming ,  Franz Weber ,  Wolfgang Siegel ,  Hartwig Schroeder ,  Stephan Freyer ,  Peter Oedman

IPC分类号： C07D493/04 ,  C12P17/18 ,  A01N43/40

摘要： The invention pertains to processes to produce dry biomass of pyripyropene producer organisms, processes to obtain pyripyropenes from such dry biomass, as well as to processes to produce compounds of Formula III and/or Formula IV and/or Formula V from the pyripyropenes obtained from the dry biomass. The invention does further pertain to the dry biomass itself, as well as processes using said dry biomass to obtain pyripyropenes for the production of compounds of Formula III and/or Formula IV and/or Formula V, including processes using said dry biomass to obtain pyripyropenes or compounds of Formula III and/or Formula IV and/or Formula V in order to produce pest control compositions, in particular insecticides, comprising such compounds.