An embodiment of the present invention provides a separator for a non-aqueous secondary battery containing a porous substrate; a heat-resistant porous layer that is provided on one side or on both sides of the porous substrate, and that contains a binder resin and inorganic particles, the inorganic particles having an average primary particle diameter from 0.01 μm to less than 0.45 μm; and an adhesive layer that is provided on one side or on both sides of a laminated body of the porous substrate and the heat-resistant porous layer, and adhesive resin particles adhered to the laminated body.