Provided are a display panel, a preparation method thereof, and a display apparatus. The display panel includes a first substrate and a second substrate disposed oppositely, and a liquid crystal layer sandwiched between the first substrate and the second substrate, wherein the first substrate includes a black matrix layer and a color filter layer which are sequentially disposed on a first base substrate; at least one of the first substrate and the second substrate further includes a spacer; and the black matrix layer includes at least one first black matrix, and an orthographic projection of each first black matrix on the first base substrate covers an orthographic projection of the spacer on the first base substrate.