A cell culture apparatus is provided. A cell culture apparatus according to one exemplary embodiment of the present invention comprises: a cell culture part comprising an accommodation space in which a plurality of supports for cell culture are disposed; a medium supply part for storing a predetermined amount of medium to be supplied to the cell culture part, and maintaining a predetermined carbon dioxide concentration of the medium stored therein by using carbon dioxide introduced from the outside through a gas supply port; and a pump, which interconnects the cell culture part and the medium supply part so as to circulate, in the cell culture part, a medium stored in the medium supply part.